90 (p = 0.113, I2 = 0.438).

Conclusion: IGRA is negative impacted by IST. Current guidelines suggesting TB screening before anti-TNF therapy may be inadequate in patients already on IST. Latent TB testing appears best performed prior to the initiation of IST in IMID patients. Key Word(s): 1. inflammatory disease; 2. crohn’s disease; 3. ulcerative colitis; 4. tuberculosis; Presenting Author: SHINJI www.selleckchem.com/products/ch5424802.html SATO Additional Authors: HIDENARI NAGAI, HIROSHI MORITA, HIDENORI KURAKATA, YASUKIYO SUMINO, YOSHINORI IGARASHI Corresponding Author: SHINJI SATO Affiliations: Toho University Omori Medical Center Objective: Evaluation of bile acids (BA) is a useful method for assessing changes of the intestinal flora in patients with ulcerative colitis (UC). During enterohepatic circulation, conjugated BA is deconjugated to free BA by intestinal bacteria. The presence of intestinal microflora (Clostridium and Eubacterium) leads to 7α-dehydroxylation of cholic acid (CA) and chenodeoxycholic acid (CDCA), yielding deoxycholic acid (DCA) and lithocholic acid, respectively. It was reported that the Lachnospiraceae subgroup of Firmicutes (including Clostridium) are decreased in the colon R428 of UC patients compared

to controls without inflammatory bowel disease. We have already reported that the serum %CDCA is significantly higher in patients with UC than in healthy volunteers (HV), while serum %DCA is significantly lower in UC patients than in HV, and these changes 上海皓元医药股份有限公司 do not depend on the activity or extent of UC. The aim of the present study was to elucidate the effects of daikenchuto (DKT) in patients with UC by examining the serum BA profile. Methods: The study population was 10 patients in whom UC was diagnosed from endoscopic and histological findings. All patients underwent ileocolonoscopy with appropriate biopsies. Treatment was given with mesalazine or salazosulfapyridine (5-ASA) and all patients

achieved remission. After entering remission, they were treated with by 5-ASA plus the DKT (7.5–15.0 g/day) for 4 weeks. The control group was composed of 8 HV. Routine laboratory tests were performed on the basis of clinical need, while fasting serum samples for measurement of BA were obtained before and after treatment with 5-ASA plus DKT for 4 weeks. Serum BA fractions were analyzed by HPLC. Results: There were no significant differences of serum total BA among the 2 groups. Before treatment, %CDCA was significantly higher in the UC groups than in the HV group, while %DCA was significantly lower than in the HV group. In the UC group, %CDCA was significantly lower and %DCA was significantly higher after 4 weeks of DKT treatment. There were no significant differences in the ratio of conjugated BA to total serum BA among the 2 groups.