5A). In contrast, there were no significant differences in the level of Ang-2 proteins between sham-operated and ME rats on day 7 (Fig. 5B). Although the level of Ang-2 protein of vehicle-injected ME rats was increased compared with that of age-matched sham-operated rats on day 28, there were no significant differences in the level of Ang-2 protein between vehicle-injected and NPC-injected ME rats on day 28 (Fig. 5B). Figure 5 Effect of injection of neural progenitor cells (NPCs) on the levels of angiopoietin-1 (Ang-1) (A) and Ang-2 (B) proteins after cerebral

embolism. Bands Inhibitors,research,lifescience,medical corresponding to Ang-1 and Ang-2 of sham-operated (sham), microsphere-induced cerebral embolism (ME), … Effect of Inhibitors,research,lifescience,medical injection of NPCs on the levels of Tie2 in brain capillaries The Ang-1/2 receptor Tie2 is expressed predominantly in vascular endothelial cells. Therefore, we examined the effect of the NPCs on the levels of Tie2 proteins in brain capillaries of sham-operated and vehicle- or NPC-injected rats on days 7 and 28 after surgery. The level of Tie2 proteins was decreased on day 7 after the embolism but returned to the level

of sham-operated rats on day 28 (Fig. 6). NPC injection tended to increase the level of Tie2 protein compared with that of vehicle-injected ME rats on day 28 (Fig. Inhibitors,research,lifescience,medical 6). Figure 6 Effect of injection of neural progenitor cells (NPCs) on the level of Tie2 protein in brain capillaries after cerebral embolism. Bands corresponding Inhibitors,research,lifescience,medical to Tie2 of sham-operated (sham), microsphere-induced cerebral embolism (ME), vehicle-injected ME (Veh-ME), … Effect

of injection of NPCs on the levels of occludin and ZO-1 in brain capillaries We further examined the level of the tight junctional proteins occludin and ZO-1, which are found in the brain capillaries of the blood–brain barrier. The level of occludin was decreased compared with that in age-matched Inhibitors,research,lifescience,medical sham-operated rats on days 7 and 28 after surgery (Fig. 7A). Injection of NPCs tended to attenuate the decrease in the level of occludin on day 28 after the embolism, although there was no significant difference (Fig. 7A). The level of ZO-1 was also decreased on day 7 after the embolism and remained at that value up to day 28 (Fig. 7B). The injection of NPCs tended to inhibit the reduction in the amount of ZO-1 protein in brain capillaries on day 28 after the embolism (Fig. 7B). Figure 7 Effect CYTH4 of injection of neural progenitor cells (NPCs) on the levels of occludin (A) and ZO-1 (B) proteins in brain capillaries after cerebral embolism. Bands corresponding to occludin and ZO-1 of sham-operated (sham), microsphere-induced cerebral embolism … Effect of injection of NPCs on the localization of Ang-1 and MGCD0103 phenotype of injected NPCs The expression of Ang-1 in vehicle- and NPC-injected ME rats on day 28 after the embolism was examined histologically.