Prior to appetitive training (Pavlovian, instrumental and transfer sessions), rats were food restricted to 85% of their ad-libitum weight and maintained this weight. During the 14 days of cocaine self-administration training,

rats were allowed ad-libitum access to food but were allowed 30 min access to water following each session. For the reacquisition transfer sessions, rats were returned to the food-restricted diet (85%ad libitum) with free access to water. After Pavlovian and instrumental training, but prior to cocaine self-administration, rats were prepared for surgery as in Experiment 1. All rats were implanted with a custom-made chronic indwelling catheter into their right jugular vein under aseptic conditions. Catheter construction and surgical implantation have been described previously (Carelli & Deadwyler, 1994). During the same surgery, a subset of rats (n = 9) high throughput screening were then chronically implanted with bilateral electrophysiological arrays aimed at the NAc core in one hemisphere and the NAc shell in the contralateral hemisphere, Linsitinib price as described in Experiment 1. Two rats were prepared for self-administration but

did not receive arrays. All rats were allowed at least 7 days to recover before self-administration training. Rats were run in two different contexts. For appetitive training (Pavlovian, instrumental and transfer sessions), rats were run in the same behavioral test chambers as described in Experiment 1, except that an infrared beam (MED Associates) was positioned on either side of the foodcup to allow precise detection of the timing of foodcup entries

and exits. For cocaine self-administration, rats were trained in a separate context in another room in the laboratory. These smaller test chambers (25 × 25 × 30 cm; MED Associates) were comprised of two clear Plexiglas walls in the front and rear, and two stainless-steel walls on the left and right side of the chamber. Each behavioral chamber was housed in a larger sound-attenuating cabinet equipped with CYTH4 a fan to mask noise. Unlike the solid plastic floor in the appetitive test chambers, the floorgrid in these contexts was comprised of evenly-spaced stainless-steel bars (0.5 cm diameter, 1.5 cm apart). On the left wall a centrally-located houselight was positioned 1 cm below the Plexiglas ceiling. On the right wall, 5 cm below the ceiling, two jewel lights were spaced 14 cm apart. An illuminated nosepoke hole (2.5 cm diameter) was located 1 cm above the floorgrid in the middle of the left wall, and a recessed foodcup was located on the opposite wall. Cocaine was administered via an intrajugular catheter attached to a syringe. Cocaine infusion was controlled via a motor-driven syringe pump (MED Associates), and tubing was tethered using a counterweighted arm to provide for animal mobility. Pavlovian training.