Table 1 provides a summary of the relationships among the levels of various inflammatory mediators throughout the protocol. The results demonstrated significant correlations among selected mediators, with CRP levels being independent of the other inflammatory biomarkers. However, the acute phase reactants, BPI and LBP, showed the most consistent relationship as markers of systemic inflammation throughout the pregnancy and ligature-induced disease. Table 2 provides a similar assessment see more in relating the inflammatory mediators to serum antibody levels to oral bacteria at baseline, mid-pregnancy and delivery.

Using a forward stepwise regression to assess the level of antibody specificity that best predicted the individual systemic inflammatory analyte levels, the results provided some interesting outcomes. Generally PGE2, RANTES and BPI levels were unrelated to the antibody responses. CRP, IL-8, MCP-1 and LBP showed significant relationships to Panobinostat antibody response profiles at baseline. IL-8 and MCP-1 levels maintained a relationship to

specific antibody profiles through mid-pregnancy, including both antibody specificities and direction. IL-6 levels were related to specific antibody patterns at mid-pregnancy and delivery. Examination of the overall systemic antibody responses indicated that responses to F. nucleatum, P. gingivalis, A. actinomycetemcomitans and C. rectus were associated most frequently with variations in inflammatory mediator levels. Finally, we attempted to model the oral clinical disease expression via a specific profile of systemic inflammatory responses. The results in Table 3 provide a summary of these analyses. Levels

of IL-6, IL-8 and LBP demonstrated a significant profile across the entire population irrespective of the sampling interval. Separating these comparisons into the individual sampling times demonstrated that none of the analytes profiled the oral clinical presentation at baseline. However, at mid-pregnancy, PGE2 and RANTES were related significantly to the oral disease, with only PAK5 BPI levels as a significant correlation of oral disease at delivery. Interestingly, throughout the experimental protocol, the profiles of serum mediators were less dependent upon the sampling interval, and patterned more closely the actual clinical presentation of the animals throughout the course of the study (Table 3). As more attention is being directed towards chronic diseases in the human population, new concepts of aetiology and resulting loss of tissue/organ function continue to develop. Many of these chronic diseases have been identified to have components of chronic inflammation, both locally and systemically, that contribute to inducing collateral damage of the host resulting in the clinical symptoms.