The assessment at age 5 also provided an opportunity to confirm the previous Detroit finding that elicited play provides GDC-0068 an early indicator of effects of prenatal alcohol exposure on verbal ability in childhood. The aims of this study are to: (1) examine which aspects of the infant’s social environment appear to most strongly influence the early development of symbolic play; (2) test the hypothesis that, as in Detroit, prenatal alcohol exposure will be specifically associated with poorer competence in symbolic play, as indicated by the elicited play measure; (3) examine the degree to which symbolic play in infancy is predictive of verbal competence at 5 years of age; and

(4) examine the degree to which infant symbolic play can be used to discriminate infants subsequently diagnosed at 5 years as having FAS or alcohol deficits from those who were heavily alcohol-exposed but did not meet criteria for the syndrome. The sample of 107 infants (57 boys and 50 girls) and their mothers was drawn from a cohort PI3K inhibitor of 159 Cape-Colored women

living in Cape Town, South Africa, who are participating in a prospective study on the effects of heavy prenatal alcohol exposure on neurobehavioral development. The mothers were recruited between July 1999 and January 2002 from the antenatal clinic of a midwife obstetric (MOU) unit that serves an economically disadvantaged Cape-Colored community (Croxford & Viljoen, 1999). The sample includes 66 heavy drinking mothers and 41 light drinkers and abstainers who were recruited during the same period by our research nurse. Antenatal care was initiated at 19.1 weeks gestation on average (range = 6.0–34.0 weeks). Each mother was interviewed during her initial antenatal visit to the MOU regarding her alcohol consumption both at the time of conception and at the time of recruitment, using an interview derived from the timeline follow-back approach (Sokol, Martier, & Ernhart, 1985) used in the Detroit Longitudinal Alcohol Exposure Study (Jacobson, Chiodo, Sokol, & Jacobson, 2002). Any woman averaging at least 1.0 oz of absolute alcohol (AA) per day (AA/day),

the equivalent of two standard drinks, or reporting at least two binge drinking episodes (five standard drinks per occasion) during the first trimester of pregnancy was invited to participate in the study. Oxymatrine Women initiating antenatal care at this clinic who drank less than 0.5 oz AA/day and did not binge drink during the first trimester were invited to participate as abstainers/light drinkers. Women <18 years of age and those with diabetes, epilepsy, or cardiac problems requiring treatment were not invited to participate. Religiously observant Moslem women were also excluded because their religious practices prohibit alcohol consumption. Infant exclusionary criteria were major chromosomal anomalies, neural tube defects, multiple births, and seizures.