2 Longibrachiatum Clade. Cultures grown on PDA. a, b T. aethiopicum, G.J.S. 10–165. c T. capillare, G.J.S. 10–170. d T. effusum, DAOM 230007. e, f T. flagellatum, G.J.S. 10–162. g, h T. gracile, G.J.S. 10–263, just beginning to sporulate. i. G.J.S. 99–17. All grown 1 week at 25°C under light, except b, e, h, which were grown 1 week at 35°C in darkness with intermittent light. Note the increased sporulation in colonies grown at 35°C when compared to the same strain grown at 25°C (b vs. a, e vs. f) Fig. 3 Longibrachiatum Clade. Cultures grown on PDA. a–c Hypocrea orientalis (a G.J.S. 06–317, b G.J.S. 04–321, c G.J.S. 04–316, reverse showing diffusing yellow pigment). d T. parareesei G.J.S.

04–41. e, f T. pinnatum (e G.J.S. 04–100, f G.J.S. 02–120). g T. saturnisporopsis Tr 175. h, i T. solani G.J.S. 08–81 (h colony from above, i colony reverse) Fig. 4 Trichoderma aethiopicum. a, b Pustules on SNA. c–g Conidiophores MLN2238 clinical trial from SNA (Arrows in d, g show intercalary phialides). h, i Conidia. j Chlamydospores. All from SNA. a, b, d, e, h, j from G.J.S. 10–167; c, g from 10 to 166; f, i from G.J.S. 10–165. Scale bars: a = 0.5 mm, b = 100 μm, c–e, j = 20 μm,

f–i = 10 μm MycoBank MB 563902 Trichodermati longibrachiato Rifai et T. pinnato Samuels simile sed ob conidiorum longitudinis Fer-1 solubility dmso ad latitudinem rationem majorem, 1.4–1.5, distinguendum. Holotypus: BPI 882291. Optimum temperature for growth on PDA and SNA 25–35°C; after 96 h in darkness with intermittent light colony on PDA and SNA completely filling a 9-cm-diam Petri plate. Conidia forming within 24 h at 35°C and after 48 h at 25 and 30°C on PDA in darkness (only sparingly produced on PDA incubated 1 week under light); diffusing yellow pigment forming at 25, 30 and 35°C

within 24 h; surface mycelium disposed in rays; at 35°C conidia covering nearly the entire colony. Conidia remaining white for a long time, slowly becoming dark green. Colonies grown on SNA in darkness with intermittent light forming conidia within 72–96 h at 30 and 35°C; conidia forming at 25°C in light within 10 day. On Meloxicam SNA conidia forming in minute pustules, < 0.25 mm diam, individual conidiophores visible within pustules; pustules formed of intertwined hyphae. Conidiophores terminating the ends of hyphae in pustules, typically comprising a long axis with phialides produced directly or shorter or longer branches arising from the conidiophore and producing phialides directly or rebranching, new branches producing phialides directly. Sterile hairs not formed. Intercalary phialides common (Fig. 4d, g). Phialides (n = 90) cylindrical to lageniform, (3.0–)5.7–9.5(−12.7) μm long, (1.7–)2.2–2.7(−3.2) μm at the widest point, L/W (1.2–)2.2–4.2(−6.2), (1.0–)1.5–2.0(−2.5) μm wide at the base, arising from a cell (1.5–)1.7–2.5(−3.7) μm wide.