Margin status and length were measured by a single uropathologist. Biochemical recurrence was defined as serum prostate specific antigen greater than

0.1 ng/ml on 2 consecutive tests. Cox regression models were constructed to evaluate predictors of biochemical recurrence.

Results: Of 1,398 consecutive patients who underwent robotic prostatectomy Selleck Danusertib positive margins were present in 243 (17%) (11% of pathological T2 and 41% of T3). Preoperative prostate specific antigen, pathological stage, Gleason score, margin status, and margin length as a continuous and categorical variable (less than 1, 1 to 3, more than 3 mm) were independent predictors of biochemical recurrence. Patients with negative margins and those with a positive margin less than 1 mm had similar rates of biochemical recurrence (log rank test p = 0.18). Surgical margin location was not independently associated with biochemical recurrence.

Conclusions: Margin status and length are independent predictors of biochemical recurrence following robotic radical prostatectomy. Although longer followup and validation studies are necessary for confirmation, patients with a positive margin less than 1 mm appear to have similar recurrence

rates as those with negative margins.”
“In vitro studies suggested that nitrite may play a cytoprotective role in inflammation. The aim of the present clinical study was to investigate the Niraparib ic50 relationship between the NO

metabolites nitrite and nitrate and the biomarkers of oxidative stress 3-nitrotyrosine (3-NT) and 15(S)-iso-PGF(2 alpha) in patients suffering from chronic inflammatory rheumatic diseases. In morning urine from 28 patients with different chronic inflammatory rheumatic diseases (23-82 years of age) and from 41 healthy persons of both genders, nitrite and nitrate were quantitated by GC-MS, and 3-NT and 15(S)-iso-PGF2a by GC-MS/MS. Mean creatinine-corrected urinary excretion rates of nitrite (1.1 versus 0.19 pmol/mmol, P= 0.00012) and 3-NT (1.2 versus 0.39 nmol/mmol, P= 0.01629), but not of nitrate (105 versus 106 pmol/mmol), were significantly elevated in rheumatism Calpain as compared to health. Urinary excretion rate of 15(S)-iso-PGF2a did not differ between patients and healthy subjects (65 versus 69 pmol/mmol creatinine, P=0.48). In rheumatism, urinary 3-NT correlated closely with nitrite (R=0.788, P<0.0001) and moderately with nitrate (R=0.45, P<0.016), but did not correlate with 15(S)-iso-PGF(2 alpha) (R=-0.083, P=0.68). In healthy persons there was no correlation between urinary 3-NT and nitrite or nitrate. Our study suggests that urinary nitrite may represent a novel specific biomarker of nitrative stress in chronic inflammatory rheumatic disease. In another eight patients with chronic inflammatory rheumatic diseases we found higher nitrite concentrations in synovial fluid as compared to serum (1.30 versus 0.35 mu M).