[10] To explore the relevance of this signaling pathway for contr

[10] To explore the relevance of this signaling pathway for control of HCV RNA replication in mouse liver-derived cells, we generated stable liver cell lines of WT mice and knockout animals with targeted disruption of MAVS,−/−, IRF3,−/−, or IFNAR−/− by in vivo immortalization as described in detail in the Materials and Methods section. In brief, animals were subjected to hydrodynamic http://www.selleckchem.com/products/ABT-263.html tail vein injection of transposon plasmids for expression of constitutively active Akt1 (myrAkt1), for mutated Kras (Kras-G12V), and a short hairpin RNA (shRNA) targeting mouse p53 (shRp53) together

with a plasmid encoding a sleeping beauty transposase (pPGK-SB13) to facilitate genomic integration of the transferred transposons. This treatment led to the growth of palpable liver tumors ∼6-10 weeks postinjection. At this timepoint, animals were sacrificed and liver tumors were collected to establish individual cell lines by limiting dilution subcloning. Established mouse liver tumor (MLT) cell lines exhibited robust and sustained

cell growth in cell culture (Fig. 1A). Genetic disruption of cognate innate immune signaling molecules was confirmed by PCR (data not shown). Overexpression of myrAkt1 and Kras-G12V induces HCC as well as cholangiocellular carcinomas (CCC), which may originate from hepatocytes.[11, 12] Although hydrodynamic injection mainly targets hepatocytes,[13] we characterized the MLT-MAVS−/− cell line by subcutaneous LEE011 datasheet implantation

and subsequent immunohistochemical analysis of induced tumors growing in recipient mice. Using this approach, we confirmed expression of HCC markers cytokeratine 8 (CK8) and CK18, whereas CK19, a marker of cholangiocarcinoma cells, was not expressed (Supporting Fig. S1). Since miR-122 is check details an important determinant of HCV tissue tropism and enhances HCV RNA-translation/replication in MEFs,[6, 7] we determined endogenous levels of mouse miR-122 in these novel liver cell-derived cell lines (Fig. 1B). The abundance of miR-122 was more than 1,000-fold lower in all generated cell lines compared with primary mouse hepatocytes (PMHs), which expressed high endogenous levels of miR-122 comparable to that observed in primary human hepatocytes and ∼3-5-fold lower compared with the level in mouse and human liver. Next we explored the relevance of innate immune signaling and miR-122 expression for HCV RNA replication in these cells by transfecting them with a JFH1 luciferase reporter replicon (Pol +). A defective replicon with an inactivating mutation of the NS5B RNA-dependent RNA-polymerase (Pol −) served as negative control. As expected, we observed efficient amplification of the replication competent replicon in the highly permissive human hepatocarcinoma cell line Huh-7.

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