, 2001; Boehm, 2003; Liu et al., 2006 and Thupaki et al., 2010). Recreational beach use, especially in California (where surfing is common), is not limited to the shoreline. This

makes it Natural Product Library purchase important to evaluate FIB contamination and the processes controlling it over wider recreational domains where physical processes are different, and FIB survivorship may also change (Davies-Colley et al., 1994 and Kim et al., 2004). Here we present results from an along and cross-shore resolved field program with joint physical and bacterial observations designed to identify the dominant mechanisms controlling FIB variability within (and seaward) of the surfzone. By directly measuring currents out to 300 m cross-shore, we both enable the evaluation FIB flow fields PTC124 cost over appropriate recreational domains, and avoid estimating current velocity from wave direction or alongshore drift, which has increased uncertainty in other models (Boehm, 2003; Kim et al., 2004). In the present paper we focus on quantifying the contribution of physical processes (advection and diffusion) to observed FIB patterns, and developing a best-fit physical model from this analysis. The contribution of biological processes to nearshore FIB variability is addressed in Rippy et al. (2012). Southern California’s Huntington State Beach is ∼3.2 km long, with chronically poor surfzone water

quality (Grant et al., 2001 and Kim

et al., 2004). At its southern end, the beach receives brackish flows from the Talbert Marsh (TM) and the Santa Ana River (SAR), both of which have been implicated as sources of surfzone FIB (Kim et al., 2004). In fall 2006, a multi-institutional field campaign (“HB06”) focused on observing nearshore waves, currents, temperature, phytoplankton, and FIB at this beach. The present study concerns the bacterial component of HB06, a 5-h FIB survey with high spatial and temporal resolution conducted on October 16th along transects extending 1 km north of the TM/SAR outlets, and 300 m offshore. FIB concentrations were measured at 8 stations: 4 in knee-deep water along a 1000 m alongshore transect north of SAR (SAR, TM, FHM, F1; Fig. 1), and 4 along a 300 m cross-shore transect starting at F1 (knee-deep Cetuximab mw water), and terminating at an offshore Orange County Sanitation District mooring (OM) in ∼8 m mean water depth (F1, F3, F5, F7, OM; Fig. 1). Every 20 min, from 0650 h to 1150 h PDT, 100 ml water samples were taken at all stations. Samples were stored on ice and transported to the Orange County Sanitation District (OCSD) within 6 h of collection. All samples were analyzed for Escherichia coli (IDEXX Colilert) and Enterococcus (EPA method 1600) concentrations by OCSD personnel. Temporal rates of FIB loss were estimated for each station from regressions of log (FIB) versus time.