Additionally, we found that HIF-1α overexpression diminished VEGF production, whereas only AdHIF-2α transduction resulted in elevation of VEGF expression. Therefore, it seems that two isoforms of HIF may play a distinct role in regulation of VEGF production in porcine proximal tubular epithelial cells, which are the major target of OTA action. Moreover, only HIF-2 exerts protective effect, especially against short-term acute kidney injuries. These results are in accordance with studies showing that HIF
may be protective in acute renal injuries whether in case of chronic ones they exert opposite effect (Manotham et al., 2004). Still, the role of each HIF Romidepsin purchase isoform in different kidney cell types may be various. Additionally, also the other factors, such as AP-1 and SP-1, should be investigated in this context. In conclusion, we have shown complicated pattern of VEGF regulation by different toxins affecting kidney biology. To our knowledge, the influence of AAI and OTA on some transcription factors have not been investigated before and further investigations are necessary to analyze this intriguing effects. The author declares that there are no conflicts of interest. This work was supported by grants from Polish Ministry for Science and Higher Education (Nos.: N N401 297835 and N N301 033440). The Faculty of Biochemistry, Biophysics and Biotechnology of the Jagiellonian
University is a IGF-1R inhibitor beneficiary of the structural funds from the Clomifene European Union and the Polish Ministry of Science and Higher Education (Grants Nos.: POIG.02.01.00-12 064/08, POIG 01.01.02-00-109/09, POIG.02.02.00-014/08 and 01.01.02-00-069/09). A.J. is a recipient of the Wellcome Trust International Senior Research Fellowship in Biomedical Science. A.L. is a recipient of Fellowship for Young Scientists funded by Ministry of Science and Higher Education. “
“Fluoxetine (FLX) is a selective serotonin reuptake inhibitors (SSRIs) with controversial
effects on carcinogenesis, that was reported to be ineffective against aggressive T-cell lymphoma in nude athymic mice, despite the significant decrease of such tumors in BALB/c mice, in which it possibly acted on immune system to inhibit tumor growth (Frick et al., 2008). However, it has been shown to enhance apoptosis and control cell cycle in Burkitt lymphoma, in spite of not affecting the viability of non-tumor peripheral blood mononuclear cells (Serafeim et al., 2003). Meanwhile, FLX has been reported to promote metastasis formation in young transplanted melanoma mice (Kubera et al., 2009). Once FLX is orally administered, it has a direct contact with the epithelia in the gastrointestinal tract (Arimochi and Morita, 2006), inducing an increase of serotonin (5-HT) levels by the blockade of serotonin reuptake transporter (SERT) (Bertrand et al., 2008).