After 30 min incubation in TBS-T containing the secondary antibody (1:800 dilution of goat https://www.selleckchem.com/products/mm-102.html IgG against rabbit IgG, Sigma) conjugated with alkaline phosphatase, the membrane was washed twice with TBS-T and revealed by NBT/BCIP color reagent using standard procedures. Acknowledgements JCA was supported by a grant from the French Ministry of Education and Research. Financial support came from the Centre National de la Recherche Scientifique, the Agence Nationale de la
Recherche (ANR 07-BLAN-0118 project) and the Université de Strasbourg. This work was done in the frame of the Groupement de Recherche (GDR2909-CNRS): « Métabolisme de l’Arsenic chez les Micro-organismes». Electronic supplementary material Additional file 1: Supplemental table S1. Selected genes differentially expressed after 8 hours arsenite stress. (PDF 167 KB) Additional file 2: Supplemental table S2. Oligonucleotides used in the study. A. Identification of transposon insertion sites in H. arsenicoxydans mutants. B. Quantitative RT-PCR. (PDF 68 KB) References
1. Mead MN: Arsenic: In search of an antidote to a global poison. Environ Health Perspect 2005, 113:A378-A386.PubMedCrossRef 2. Rosen BP: Biochemistry of arsenic detoxification. FEBS Lett 2002, 529:86–92.PubMedCrossRef 3. Smith AH, Lingas EO, Rahman M: Contamination of drinking-water by arsenic in Bangladesh: A public health emergency. Bull World Health Organ 2000, 78:1093–1103.PubMed 4. Muller D, Simeonova DD, Riegel P, Mangenot S, Koechler S, Lièvremont VX-680 supplier D, Bertin PN, Lett MC: Herminiimonas arsenicoxydans sp. nov., a metalloresistant bacterium. Int J Syst Evol Microbiol 2006, 56:1765–1769.PubMedCrossRef 5. Carapito C, Muller D, Turlin E, Koechler S, Danchin A, Van Dorsselaer A, Leize-Wagner E, Bertin PN, Lett MC: Identification of genes and proteins involved in the
pleiotropic response to arsenic stress in SB431542 supplier Caenibacter arsenoxydans , a metalloresistant beta-proteobacterium with an unsequenced genome. Biochimie 2006, 88:595–606.PubMedCrossRef 6. Muller D, Medigue C, Koechler S, Barbe V, Barakat M, Talla E, Bonnefoy MRIP V, Krin E, Arsene-Ploetze F, Carapito C, et al.: A tale of two oxidation states: bacterial colonization of arsenic-rich environments. PLoS genetics 2007,3(4):e53.PubMedCrossRef 7. Weiss S, Carapito C, Cleiss J, Koechler S, Turlin E, Coppee JY, Heymann M, Kugler V, Stauffert M, Cruveiller S, et al.: Enhanced structural and functional genome elucidation of the arsenite-oxidizing strain Herminiimonas arsenicoxydans by proteomics data. Biochimie 2009, 91:192–203.PubMedCrossRef 8. Alvarez-Martinez CE, Lourenço RF, Baldini RL, Laub MT, Gomes SL: The ECF sigma factor sT is involved in osmotic and oxidative stress responses in Caulobacter crescentus . Mol Microbiol 2007, 66:1240–1255.PubMedCrossRef 9. Muller D, Lièvremont D, Simeonova DD, Hubert JC, Lett MC: Arsenite oxidase aox genes from a metal-resistant beta-proteobacterium. J Bacteriol 2003, 185:135–141.PubMedCrossRef 10.