All rats had been fasting but permitted accessibility to water ad libitum for even more than 12 h before blood sample collecting. Blood samples had been collected into two tubes,heparinized centrifuge tubes and dry non heparinized centrifuge tubes. The heparinized blood was applied for any hematological determination, which contain red blood cell hemoglobin hematocrit mean corpuscular volume indicate corpuscular HGB indicate corpuscular HGB concentration red cell distribution width platelets imply platelet volume platelet distribution width white blood cell counts, reticulocytes neutrophils lymphocytes monocytes eosinophils and basophils by using a MEK 7222K Hematology analyzer in accordance to the suppliers operator manual. Plasma was isolated and employed to determine the prothrombin time making use of a coagulometer The non heparinized blood was allowed to coagulate before becoming centrifuged as well as the serum separated.
Serum was analyzed for alterations in biochemistry making use of an automatic biochemical dig this analyzer which measured aspartate aminotransferase action alanine aminotransferase action alkaline phosphatase action albumin total protein complete cholesterol creatine phosphokinase activity complete bilirubin direct bilirubin creatinine triglycerides urea and glucose The concentrations of sodium ions potassium ions and chloride ions have been measured with an electrolyte autoanalyzer All parameters of blood chemistry and hematology had been measured following normal procedures. Organ weights, gross necropsy and histopathological examination All animals have been fasted overnight just before scheduled necropsies. A plete necropsy was performed on all animals and consisted of an external examination, which include the identification of all clinically recorded lesions, and also a comprehensive internal examination.
The rats were anesthe tized with diethyl ether and sacrificed by decapitation following blood assortment through the abdominal aorta. Immediately after dissection to remove unwanted fat and connective tissue, the following organs had been meticulously dissected out and weighed,brain thymus glands, heart, lungs, liver, spleen, kidneys, adrenal glands, testis ovary and epididymis uterus. The relative organ read full article excess weight was calculated depending on the terminal body bodyweight ahead of fixation. The following organs of all animals have been fixed in 10% neutral formalin,brain, thoracic spinal cord, thyroid glands, Gleys glands, pituitary gland, thymus, adrenal glands, esophagus, salivary glands, abdomen, small intestine sizeable intestine pancreas, spleen, lungs, trachea, kidneys, liver, heart, aorta, bladder, testes, epididymides, ovaries, uterus, prostate gland, vagina, mammary gland, sciatic nerve, urinary mesenteric lymph node, sternum, sub mandibular lymph nodes, bone marrow.