Authors’ contributions AB designed portions of the study, conducted all the experiments, and wrote the manuscript. JACH analyzed and interpreted data and critically revised the manuscript. MSF participated in data VRT752271 cost analysis. ANH coordinated the project, designed portions of the study, and helped draft and revise the manuscript. All authors have read and approved the final manuscript.”
“Background Sinorhizobium meliloti is a soil-born α-proteobacterium that can enter a nitrogen-fixing symbiosis with

Medicago sativa (alfalfa) and related legumes. The establishment of the symbiosis relies on a complex molecular dialogue between the two partners that triggers two essential and overlapping steps, nodulation and infection (see [1, 2] for reviews).

During the infection process, bacteria colonize root hairs forming Infection MK5108 Threads (ITs) that extend and proliferate towards the nodule primordium that is formed in the root cortex. Ultimately, rhizobia Sotrastaurin ic50 are released from ITs within nodule cells where they fix molecular dinitrogen. Nodulation and infection are tightly controlled processes and we have shown recently that bacterial adenylate cyclases (ACs) contribute to the negative autoregulation of infection [3]. ACs (EC 4.6.1.1) are enzymes that synthesize cAMP (3′, 5′-cyclic adenosine monophosphate) from ATP. There are 6 non-homologous classes of ACs as a typical example of convergent evolution [4, 5]. Class III is the universal class whose members can be found in both prokaryotes and eukaryotes although, to our knowledge, their presence in plants has not been established [6]. The number of class III ACs strikingly varies in bacteria. E. coli has none whereas cyanobacteria, mycobacteria and rhizobia, a group of phylogenetically-diverse bacteria [7], have many, up to 32 in the soybean symbiont Bradyrhizobium japonicum. (-)-p-Bromotetramisole Oxalate The biological function of class III ACs in bacteria remains poorly understood. Class III ACs synthesize cAMP in response to environmental cues such as light, oxygen, nitrogen and pH in Cyanobacteria [8] or high osmotic pressure in Myxococcus xanthus[9, 10]. Class III

ACs are also involved in biotic interactions as they contribute to virulence in M. tuberculosis, P. aeruginosa and in some fungal pathogens [5, 11–13]. CO2 and Ca2+ are signals used by pathogens to sense their host environment through their AC–cAMP signaling systems. Candida albicans and mycobacteria express CO2-responsive ACs [5, 14] whereas CyaB from P. aeruginosa is Ca2+ sensitive. Another example of cAMP-associated signal being used by the human fungal pathogen C. albicans to sense the host environment is the bacterial peptidoglycan present in blood serum [15]. We have recently described the first instance of class III ACs contributing to a symbiotic (mutualistic) interaction, between Sinorhizobium meliloti and its host plant Medicago sativa[3]. S.