burgdorferi can utilize several sugars that may be available during persistence in the tick, including trehalose, N-acetylglucosamine (GlcNAc), and chitobiose. The spirochete grows to a higher cell density in trehalose, which is found in tick hemolymph, than in maltose; these two disaccharides differ only in the glycosidic linkage between the glucose monomers. Additionally, B. burgdorferi grows to a higher density in GlcNAc than

in the GlcNAc dimer chitobiose, both Dinaciclib cell line of which may be available during tick molting. We have also investigated the role of malQ (bb0166), which encodes an amylomaltase, in sugar utilization during the enzootic cycle. In other bacteria, MalQ is involved in utilizing maltodextrins and trehalose, but we show that, unexpectedly, it is not needed for B. burgdorferi to grow in vitro on any of the sugars assayed. In addition, infection of mice by needle inoculation or tick bite, as well as acquisition and maintenance of the spirochete in the tick vector, does not require MalQ. Borrelia burgdorferi is the spirochete that causes Lyme disease (Burgdorfer et al., 1982; Benach et al., 1983; Steere et al., 1983; Radolf et al., 2012); its enzootic cycle involves CB-839 order an Ixodes tick vector and a vertebrate host (Lane et al., 1991; Spielman, 1994; Piesman & Schwan, 2010). Following

acquisition by a feeding tick, B. burgdorferi persists for several months until transmission to a vertebrate, typically a mammal. Little is known about the physiology of the spirochete and its metabolic requirements in the two distinct environments encountered in the enzootic cycle (Gherardini et al., 2010). Disaccharides and oligosaccharides may serve as carbon and energy sources for B. burgdorferi Adenosine triphosphate in vivo. Trehalose, an α(11)α glucose disaccharide, is found in tick hemolymph (Barker & Lehner, 1976). Chitobiose, a β(14)-linked dimer of N-acetylglucosamine (GlcNAc) monomers, also may be available to the spirochete during the chitin rearrangement that occurs as the tick molts; B. burgdorferi can utilize chitobiose in vitro (Tilly

et al., 2001). Escherichia coli and other bacteria can utilize maltose, an α(14) glucose disaccharide, as a carbon source (Boos & Shuman, 1998). Maltose and maltodextrins are degraded by amylomaltase, encoded by the malQ gene, and E. coli malQ mutants are unable to grow on maltose (Monod & Torriani, 1948, 1950; Wiesmeyer & Cohn, 1960a, b; Pugsley & Dubreuil, 1988). Borrelia burgdorferi has a malQ homolog (bb0166) (Fraser et al., 1997) and can utilize maltose as a carbon source (von Lackum & Stevenson, 2005). Sequence analysis suggests that MalQ in B. burgdorferi is unusual: it is missing one of four otherwise completely conserved residues (Lys instead of Arg at position 308) (Godány et al., 2008). Godány et al. (2008) purified recombinant B. burgdorferi amylomaltase (MalQ) and demonstrated the release of glucose in the dextrinyl transferase reaction with maltose as well as other maltodextrins as substrates.