For comparison and reference, the commercial kit YeaStar

Genomic DNA Kit (Zymo Research, Orange, California, USA) was used in parallel with 1 μl of crude colony lysates. Results of this comparison represented by melting curves and banding patterns are summarized in Figure 2. When comparing the initial relative fluorescence of amplified samples, the use of DNA extracted by the commercial kit resulted in higher values on average, indicating higher yields. In 8 of the 9 species studied, no marked differences in melting curves based on kit versus crude lysates were observed, although some minor differences in the relative intensity of individual bands occurred in some of the species. Only 1 of the 9 Ku-0059436 chemical structure species, namely C. glabrata, showed both markedly Selleck Fedratinib selleck chemicals different banding patterns and melting curves, indicating that the performance of McRAPD with colony lysate was suboptimal in this case compared to the commercial kit. Our experience in routine experiments shows that the initial

relative fluorescence intensity of a McRAPD sample after amplification should exceed the relative value of 15 at the standard 30% LED power as adjusted in melting protocol by user. When a sample does not meet this condition, repeating the assay including DNA extraction is strongly recommended for reliable results. Figure 1 Results of optimization of the amount of crude colony lysates added into reaction mixture. Lanes are arranged in triplicates where each

triplicate of lanes represents results obtained with the same strain. Individual lanes within each triplicate represent variable amount of crude colony lysate added into the reaction mixture, namely 0.5, 1, and 2 μl in the order from left to right. Part (A), lane 1 and 17: molecular weight marker 200-1500 (Top-Bio, Prague, Czech Republic), lanes 2-4: C. albicans ATCC 76615; lanes 5-7: C. krusei I1-CAKR-24; lanes 8-10: C. tropicalis I3-CATR9-37; lanes 11-13: C. lusitaniae I1-CALU-33; lanes 14-16: C. parapsilosis CBS 604; part (B), lane C1GALT1 1 and 14: molecular weight marker 200-1500 (Top-Bio, Prague, Czech Republic), lanes 2-4: C. pelliculosa I3-CAPE3-10; lanes 5-7: C. guilliermondii I1-CAGU2-20; lanes 8-10: S. cerevisiae I3-SACE3-37; lanes 11-13: C. glabrata I1-CAGL-32. Figure 2 Comparison of McRAPD results obtained with DNA extracted using the commercial kit YeaStar Genomic DNA Kit ( Zymo Research, Orange, CA, USA ) and using the technique of crude colony lysates. Selected strains were subjected to DNA extraction in parallel and the DNA was used for McRAPD resulting in duplicates of melting curves and duplicates of agarose gel fingerprints.