Further,

Further, http://www.selleckchem.com/products/ganetespib-sta-9090.html 221 nm (��max of METO) and 257 nm (��max of OLME) are the wavelengths at which calibration curves were prepared for both the drugs. The criteria for obtaining maximum precision[18] by this method were calculated and found to be outside the range 0.1�C2. Once the absorptivity values are determined, very little time is required for analysis, as would require determination of absorbances of the sample solution at two selected wavelengths and few simple calculations. The standard solutions of METO and OLME were scanned separately in the UV range, and zero-order spectra for METO and OLME were recorded [Figure 1]. Maximum absorbance was obtained at 221 nm and 257 nm for METO and OLME, respectively.

Linear correlation was obtained between absorbances and concentrations of METO and OLME in the concentration ranges of 5�C25 ��g/ml and 4�C20 ��g/ ml for both drugs, respectively. The linearity of the calibration curve was validated by the high values of correlation coefficient of regression. LOD and LOQ values for METO were found to be 0.30 and 0.90 ��g/ml and 2.22 and 6.74 ��g/ml at 221 and 257 nm, respectively. LOD and LOQ values for OLME were found to be 0.16 and 0.47 ��g/ml and 0.19 and 0.57 ��g/ ml at 221 and 257 nm, respectively. These data show that the method is sensitive for the determination of METO and OLME. All the regression analysis data and the summary of validation parameters for the proposed method are reported in Table 1. The recovery experiment was performed by the standard addition method. The mean recoveries were 100.90 �� 1.780 and 100.26 �� 0.

721 for METO and OLME, respectively, which indicates the accuracy of the proposed method [Table 2]. The proposed validated method was successfully applied to determine METO and OLME in their combined dosage form. The results obtained for METO and OLME were comparable with the corresponding labelled amounts [Table 3]. The relative standard deviation (RSD) values for assay of METO and OLME were found to be 1.37 and 0.81, respectively. The RSD was less than 2%, which indicates that the proposed method is repeatable [Table 4]. Figure 1 Overlay of metoprolol succinate and olmesartan medoxomil.

Table 1 Regression analysis data and summary of validation parameter of the calibration curves Table 2 Results of the recovery studies Table Cilengitide 3 Results of analysis of tablet formulation Table 4 Results of intermediate precisions CONCLUSION No interference of the excipients with the absorbance of interest appeared; hence the proposed method is applicable for the routine simultaneous estimation of METO and OLME in pharmaceutical tablet dosage forms. The proposed spectrophotometric method was found to be simple, sensitive, accurate, and precise for simultaneous determination of METO and OLME in the tablet dosage form. The method utilizes easily available and low cost solvent like distilled water for analysis of METO and OLME.

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