In this case a twofold enhancement of the fluorescence intensity is observed. Such a behavior is qualitatively different from the one demonstrated frequently for closely VX-770 cell line placed metallic nanoparticles, where a so-called hot spot can be formed, where the total fluorescence intensity can be considerably higher than for a single nanoparticle. The difference confirms that the mechanism responsible for the fluorescence enhancement observed for a hybrid nanostructure assembled from

dielectric spheres and photosynthetic complexes has another origin. Figure 2 Wide-field fluorescence image of the PCP complexes on 1.1-μm-diameter silica nanoparticles and their fluorescence intensity. (a) Wide-field fluorescence image of the PCP complexes deposited on silica nanoparticles with a diameter of 1.1 μm. Excitation wavelength was 480 nm. www.selleckchem.com/products/SP600125.html (b) Histogram of the fluorescence intensity calculated from the wide-field fluorescence image. (c) Cross section of the fluorescence intensity obtained for the three nanoparticles shown in Figure 2a.

The enhancement factor of the fluorescence depends upon the size of dielectric particles. In Figure 3, we show PX-478 nmr a dataset similar to the one discussed above, but obtained for smaller particles, having a diameter of 600 nm. In the fluorescence map (Figure 3a), we also can see ring-like emission patterns that originate from the PCP complexes placed in the vicinity of the silica spheres. Analogous analysis has been carried out for this structure in order to estimate the influence of silica nanoparticles upon the collection efficiency of the fluorescence. In this case the fluorescence map shows however substantial inhomogenities of the emission intensity of the PCP complexes away from the nanoparticles, as evidenced by the intensity histogram (Figure 3b). An intensity cross section displayed in Figure 3c features the increase of the intensity at the edges of the nanoparticles; however, the scale of the enhancement is lower than that in the case of 1,100-nm particles. Although the particle doublet shown in Figure 3c might be on the lower side of enhancement

factors measured for this structure, we have not observed cases with the increase larger than twofold. The comparison between the fluorescence images obtained for the PCP complexes deposited on 1,100- and 600-nm silica spheres suggests that the enhancement cAMP of collection efficiency could depend upon the diameter of dielectric particles, but a clear answer can be given perhaps after performing single-molecule fluorescence studies in this geometry. Figure 3 Wide-field fluorescence image of the PCP complexes on 0.6-μm-diameter silica nanoparticles and their fluorescence intensity. (a) Wide-field fluorescence image of the PCP complexes deposited on silica nanoparticles with a diameter of 0.6 μm. Excitation wavelength was 480 nm. (b) Histogram of the fluorescence intensity calculated from the wide-field fluorescence image.