Influenza Alectinib manufacturer A viruses are enveloped viruses belonging to family Orthomyxoviridae. These viruses are promising but currently under-explored vectors, which display some advantageous features to be used as live recombinant vaccines [3] and [9], such as ability to infect and activate antigen presenting cells and present high immunogenicity at mucosal and systemic levels [10]. Indeed, some noteworthy studies have demonstrated that influenza viral vectors administered by intranasal route elicit heterospecific humoral and cellular immune responses both in the mucosal compartment

and systemically [11], [12], [13] and [14]. Moreover, intranasal administration of influenza induces mucosal immunity in the intestinal and genital tracts [15] and [16]. These features indicate that influenza vectors are useful to elicit protective immune response against mucosal or food borne diseases. The Influenza A genome consists of eight negative single strand RNA segments [17]. Each segment comprise a coding region flanked by partially complementary 3′ and 5′ non-coding regions, which contain the transcription and replication signals [18], [19], [20] and [21]. In addition,

these non-coding regions as well as their adjacent coding sequences contain the influenza segments packaging signals [20], [22], [23], [24], [25] and [26]. We have developed a modified neuraminidase segment carrying a duplication of the 3′ promoter [27] and [28] that can be used for cloning and expression of foreign sequences. In the modified segment, the expression of buy AZD6738 viral neuraminidase is controlled by the external 3′ promoter, whereas any foreign sequences ALOX15 cloned into this segment is placed under control of the internally located 3′ promoter. Recombinant viruses harboring such dicistronic NA segment (NA38) and coding a foreign sequence were able to induce significant

systemic humoral and CD8+ T cell-mediated immune responses specific for the foreign sequence. These results suggest a potential use of such recombinant viruses for the development of live vaccines against intracellular pathogens [27] and [28]. The protozoan Toxoplasma gondii is an intracellular parasite spread worldwide. Acute toxoplasmosis in pregnancy is a major cause of prenatal malformations and abortion. In immune-compromised hosts, the reactivation of chronic infections results in blindness and encephalitis with high mortality risk [29] and [30]. T. gondii infections elicit potent and long-lasting cell-mediated immune responses, in which CD8+ T lymphocytes are considered major effectors responsible for controlling parasite replication in chronic phase, mostly by secreting IFN-γ and exerting cytotoxic effect on infected cells [31] and [32].