The color intensity on the heat map correlates to the intensity (

The color intensity on the heat map correlates to the intensity (log ratio) of the expression, with red representing overexpression and green indicating reduced expression. (PDF 161 KB) Additional file 7: Quantitative RT-PCR. qRT-PCR was performed for validation of the microarray expression data. The six genes used in the experiment were smb20611, smc01505, grpE, lpiA, exoY and mcpT. Differences in gene expression

were determined by comparing the crossing points of samples measured in three replicates. Comparison of Akt inhibitor expression data was always performed between samples transferred to medium at pH 5.75 and control samples transferred to control medium at pH 7.0, 10 or 60 minutes after pH shift. In the group of genes analyzed, RpoH1-dependent, RpoH1-independent and complex regulation could be observed, in accordance to the microarray expression data. Section A includes the results obtained by qRT-PCR. The M-values

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