The highest Ms activity with the MICvalue 15.6 μg/mL was observed for compound 12 that is a 1,2,4-triazole derivative containing morpholine and pyridine nuclei as well. All the tested compounds were found to be active on yeast like fungi, Candida albicans (Ca) and Saccharomyces cerevisiae (Sc), in high concentrations with the MIC values Metabolism inhibitor of 500 or 1,000 μg/mL, whereas all compounds, except compound 8, displayed no activity against gram-negative bacterial strain. In contrast to other compounds, compound 12 demonstrated a low activity against Pseudomonas aeruginosa (Pa), a gram-negative

bacillus. Table 1 Antimicrobial activity of the compounds (μg/mL) Comp. no Microorganisms and minimal inhibition concentration Ec Yp Pa Ef Sa Bc Ms Ca Sc 3 – –

– – – – 125 1,000 1,000 4 – – – – – – 125 500 1,000 5 – – – – – – 31.3 1,000 1,000 6 – – – – – – – 500 1,000 7 – – – – – – – 500 1,000 8 62.5 62.5 62.5 31.3 31.3 62.5 125 1,000 1,000 9 – – – – – – 125 1,000 1,000 10 – – – – – – – 500 1,000 11 – – – – – – 125 500 1,000 12 – – 500 – – – 15.6 500 1,000 13 – – – – – – – 500 1,000 Amp. 8 32 >128 2 Fer-1 2 <1       Str.             4     Flu.               <8 <8 Ec: Escherichia coli ATCC 25922, Yp: Yersinia pseudotuberculosis ATCC 911, Pa: Pseudomonas aeruginosa ATCC 43288, Ef: Enterococcus faecalis ATCC 29212, Sa: Staphylococcus aureus ATCC 25923, Bc: Bacillus cereus 702 Roma, Ms: Mycobacterium smegmatis ATCC 607, Ca: Candida albicans ATCC 60193, Sc: S. cerevisiae RSKK 251, Amp.: Ampicillin, Str.: Streptomisin, Flu.: Fluconazole Almost all the compounds showed moderate-to-good urease inhibitory activity (Table 2). The inhibition Interleukin-3 receptor was increased with increasing compound concentration. Potent compound have their activities in the range of 2.37–13.23 μM. Lower IC50 values indicate higher enzyme inhibitor activity. Compound 10 proved to be the most potent showing an enzyme inhibition activity with an IC50 = 2.37 ± 0.19 μM. The least active compound 3 had an IC50 = 13.23 ± 2.25 μM.

Table 2 The urease inhibitory activity of different concentrations of KU55933 manufacturer morpholin derivatives Compounds IC50 (μM)a 3 13.23 ± 2.25 4 7.92 ± 1.43 5 6.87 ± 0.06 6 8.29 ± 2.30 7 7.01 ± 0.68 8 4.99 ± 0.59 9 8.07 ± 1.25 10 2.37 ± 0.19 11 4.77 ± 0.92 12 6.05 ± 1.19 13 4.46 ± 0.22 aMean ± SD Conclusion In this study, the synthesis of some morpholine derivatives (3–13) were performed, some of which contain an azole moiety, and their structures were confirmed by IR, 1H NMR, 13C NMR, Mass spectroscopic, and elemental analysis techniques. In addition, the newly synthesized compounds were screened for their antimicrobial and antiurease activities. Some of them were found to possess activity on M. smegmatis, C. albicans ATCC, and S. cerevisiae.