The overview of epitope mapping techniques and challenges in epitope identification has been described elsewhere [59, 60]. Although CTL and Th epitopes had representation from all nine protein-coding genes, Ab epitopes were Lenvatinib datasheet absent in the Vif, Vpr, Rev and Vpu genes. The majority of the Ab epitopes (75 out of 81) belonged to the Env gene, while the Pol gene had three and the Gag, Tat and Nef genes had one epitope each [61–65]. It should be noted that

because of the high amino acid sequence diversity of the Env gene that may differ by as much as 30% between subtypes [43], very few antibody epitopes if at all could be expected to be conserved IWR-1 research buy across a broad range of HIV-1 sequences; thus, in this study we primarily focus on CTL and T-Helper epitopes. Restricting HLA allele(s) for associated epitopes are given in Table find more 3 as per HIV Immunology database and IEDB http://​www.​immuneepitope.​org/​.

Table 2 Overview of epitopes used in the analyses. Gene Protein Total no. of epitopes Highly conserved epitopes* No of associated epitopes^     CTL # Th Ab Total CTL Th Ab Total CTL Th Ab Total Gag p17 18 32 – 50 1 – - 1 – - – -   p24 42 88 1 131 8 6 – 14 8 6 – 14   p2p7p1p6 6 18 – 24 2 – - 2 2 – - 2   Total 66 138 1 205 11 6 – 17 10 6 0 16 Pol Gag-Pol

1 – - 1 – - – - – - – -   Protease 8 – - 8 1 – - 1 1 – - 1   RT 39 20 3 62 12 1 – 13 12 1 – 13   RT-                           Integrase 1 1 – 2 1 – - 1 1 Liothyronine Sodium – - 1   Integrase 12 11 – 23 5 2 – 7 4 2 – 6   Total 61 32 3 96 19 3   22 18 3 0 21 Vif   9 2 – 11 – - – - – - – - Vpr   7 6 – 13 – - – - – - – - Tat   4 6 1 11 – - – - – - – - Rev   4 5 – 9 – - – - – - – - Vpu   1 1 – 2 – - – - – - – - Env   40 82 75 197 – - 2 2 – - 1 1 Nef   37 24 1 62 2 1 – 3 2 1 – 3   Total 229 296 81 606 32 10 2 44 30 10 1 41 # CTL epitopes included only the best-defined epitopes as described by Frahm et al. (2007) [56] * Only those epitopes present in more than 75% of the reference sequences were considered as highly conserved and thus included in the association rule mining. 3 epitopes completely overlapping with other epitopes of same type without amino acid differences were not included. ^ Associated epitopes are epitopes involved in association rules identified with a support value of 0.75 and confidence value of 0.95 Table 3 Description of the 44 epitopes used in association rule mining.