This phenomenon has been well characterized in other bacteria [64, 65], and is worthy to additional

evaluation of B. melitensis virB operon. In addition, and similar to mention for flagellar genes, microarray could detect expression of some but not all genes from an operon, due to the inherent nature of the technique. Further, our analysis method was particularly stringent in order to greatly reduce false positives at the risk of additional false negatives. Thus, other genes in the virB operon were increased in expression such as virB2, virB4, virB6, virB6 and virB11, although not statistically significant because of the stringency of our statistical analysis. Finally, genes with uncharacterized function that were differentially expressed at late-log phase compared with the stationary Cell Cycle inhibitor phase also deserve some special consideration. This group of “”hidden genes”" represents 22% of the differentially expressed genes identified in this study, and it may contain some of the heretofore unknown virulence factors utilized for B. melitensis to invade

and infect the host, as was previously suggested [24, 43, 46]. MEK162 research buy Conversely, Brucella internalization should not be disregarded as a product of synergistic action among several gene products in non-phagocytic cells. Conclusion Our study reveals that B. melitensis grown in cell culture medium at late-log phase are more invasive in non-phagocytic click here cells than cultures grown at mid-log or stationary growth phases. cDNA microarrays provide informative differential transcriptional profiles of the most (late-log growth phase) and the least (stationary growth phase) invasive B. melitensis cultures. We consider these data a platform for conducting further studies on the Brucella:host initial interaction. Since the roles of the majority of differentially expressed genes in this study are not well defined in Brucella pathogenesis, future studies on Brucella virulence

can now be specifically focused to more precisely delineate the roles of candidate genes identified in this study. Methods Bacterial strains, media and culture conditions Methocarbamol Smooth virulent Brucella melitensis 16 M Biotype 1 (ATCC 23456) (American Type Culture Collection, Manassas, VA), re-isolated from an aborted goat fetus, and its derivatives were maintained as frozen glycerol stocks. Individual 50 ml conical tubes were filled with 10 ml of cell culture medium [F12K medium (ATCC®) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (ATCC®)], inoculated with 0.1 ml (1:100 for mid-log cultures), 0.25 ml (1:40 for late-log phase cultures) and 1 ml (1:10 for stationary phase cultures) of a saturated culture of B. melitensis 16 M and incubated overnight at 37°C with 5% CO2, loose lids and shaking (200 rpm). Growth curves of cultures were determined by comparing the optical density (OD) of the culture at 600 nm with bacterial colony forming units (CFU).