We did not find a significant correlation between MYC, FBXW7, and TP53 mRNA expression. Thus, only a tendency toward correlation between an increase in MYC mRNA http://www.selleckchem.com/products/wortmannin.html ex pression and a decrease in FBXW7 mRNA expression was detected. Table 2 summarizes the associations between various clinicopathological features and the RQ of MYC, FBXW7, and TP53 mRNA expression in tumor and paired non neoplastic specimens. An increase in MYC mRNA level was associated with the presence of lymph node metasta sis and GC tumor stage III IV. A significant reduction in FBXW7 mRNA level was also associated with the presence lymph node metastasis and tumor stage III IV. Nuclear MYC protein staining is associated with intestinal type GC Positive staining for nuclear MYC and p53 was found in 64. 5% and 19.

4% of GC samples, respectively. No positivity was found for FBXW7. Table 1 summarizes the clinicopathological features and MYC and p53 immunostaining results. Expression of MYC was more frequent in intestinal type than diffuse type GC. Furthermore, MYC immunostaining was associated with increased MYC mRNA level. No association was found between p53 immunostaining and clinicopathological characteristics, TP53 copy number, or TP53 mRNA expression. Comparison of ACP02 and ACP03 cell lines Both ACP02 and ACP03 cells contained three MYC copies and only one FBXW7 copy. The number of TP53 copies was undetermined in both cell lines. Compared with mRNA expression in ACP03 cells, ACP02 cells expressed a higher level of MYC and lower levels of FBXW7 and TP53 mRNA.

Western blot analyses revealed that MYC expression was significantly higher in ACP02 cells than ACP03 cells. Moreover, FBXW7 expression was significantly lower in ACP02 cells than ACP03 cells. How ever, there was no significant difference in p53 expression between the cell lines. Immunofluorescence analysis Carfilzomib of both proteins showed a punctiform pattern of labeling, supporting the Western blot results showing an increase in MYC and reduction in FBXW7 expression in ACP02 cells compared with ACP03. Matrigel invasion assay results showed that ACP02 cells were more invasive than ACP03 cells. Migration assay results showed that fewer ACP02 cells migrated compared with ACP03 cells. Both ACP02 and ACP03 cells presented four gelatinase activity bands, MMP 9 latent, MMP 9 active, MMP 2 latent, and MMP 2 active. We found no significant differences in MMP 9 latent, MMP 2 active, and MMP 2 latent between ACP02 and ACP03 cells. However, significant differences were found between ACP02 and ACP03 cells with respect to MMP 9 active. Discussion In the current study, we observed that MYC mRNA ex pression was increased in GC samples compared with corresponding non neoplastic samples.