We prepared primary cultures of GABAergic striatal neurons and infected them with the VGLUT3-expressing

lentivirus. We measured synaptic responses with high Cl–containing internal solution and isolated the glutamatergic component of the synaptic response with bicuculline (30 μM) and/or kynurenic Epigenetic Reader Domain inhibitor acid (3 mM). As previously shown for VGLUT1 and VGLUT2 (Takamori et al., 2000 and Takamori et al., 2001), VGLUT3 expressed in GABAergic striatal neurons was sufficient to induce glutamate release in these cells that normally release only GABA (Figure 1G). After 14 days in vitro, glutamatergic EPSC were recorded in 21 of 28 infected neurons, while no glutamatergic EPSCs were recorded from uninfected control GABAergic neurons (n = 15). In addition to the analysis of evoked release, we tested whether spontaneous release of glutamate could be detected in these neurons. Blocking www.selleckchem.com/products/XL184.html GABA receptors with bicuculline (30 μM) in infected neurons revealed mEPSCs that were blocked by kynurenic acid

(3 mM, Figure 1H) and had peak amplitudes that were similar to glutamatergic neurons (Figure 1I; Moechars et al., 2006), suggesting that the transport and accumulation of glutamate by VGLUT3 in synaptic vesicles of neurons that release neurotransmitters other than glutamate is similar to transport in native glutamatergic neurons, and that VGLUT3 expression is sufficient for a glutamatergic phenotype in central neurons. Because neurons that express VGLUT1 Linifanib (ABT-869) and VGLUT2 in vivo show a correlation between the isoform expressed and probability of glutamate release (Fremeau et al., 2001 and Liu, 2003), we performed an analysis of Pvr and short-term plasticity in order to determine whether neurons

with different VGLUT isoforms release glutamate in quantitatively distinct manners. We first examined cell types in which VGLUT1 and VGLUT2 are differentially expressed in vivo. For VGLUT1 expressing cells we chose hippocampal neurons, in which 85%–90% of the excitatory synaptic current depends on VGLUT1 (Fremeau et al., 2004 and Wojcik et al., 2004). For VGLUT2 we chose thalamic neurons, in which 90% of the excitatory synaptic response depends on VGLUT2 (Moechars et al., 2006). We calculated the Pvr by comparing the charge contained in the RRP with the charge of the EPSC. Thalamic neurons had a significantly higher Pvr than hippocampal neurons and showed strong paired-pulse depression characteristic of high-release probability synapses. In contrast, hippocampal neurons showed moderate paired-pulse facilitation and lower Pvr (Figures 2A and 2B).