05). Conclusion: Normal cells and tumor cells show different cell membrane morphologies, and such morphological features provide a reliable basis for clinical pathological diagnosis and differential diagnosis of malignancies. Key Word(s): 1. AFM; 2. HCC; 3. surface scan; BGB324 nmr 4. images; Presenting Author: CHENG YAN Additional Authors: ZHANG JUN Corresponding Author: CHENG YAN Affiliations: Department of gastroenterology, the Second Affiliated Hospital f Xi’an Jiaotong University Objective: To identify gastric cardia carcinoma (GCA) associated

proteins and early intestinal metaplasia protein biomarkers. Methods: We performed navigated laser capture microdissection (LCM) to enrich the malignant (group A), Intestinal Metaplasia (IM, group B) and nonmalignant (group C) gastric cardia epithelial cells from surgical specimens of human GCA. The proteins extracted from these cells were then separated by 2-DE. Protein spots were identified by peptide mass fingerprint (PMF) based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and database searching. Results: (1) The

2-DE patterns with high resolution and reproducibility of human GCA were obtained. BVD-523 cost The mean detected number of protein spots was: 867 ± 51 in A, 836 ± 50 in B, and 905 ± 74 in C. The percent of matched spots between them was: 77.6% between A and C, 86.7% between A and B, and 79.5% between B and C. (2) Seventy two proteins associated of GCA including their cellular localization and physiological function were successfully identified. (3) Twenty three proteins were consistently differential regulated

in IM. These proteins were classified into cell proliferation and differentiation (ANXA2, ANXA4), apoptosis (Prx-2, GSTP, VDAC, BCL2L11), metabolism (ADH1C, AKR1C3, CA II, GATM, Sulfotransferase 1A1, ZFYVE1, GPR175), protease related (PCNC1), cystoskeleton (Keratin 8), chaperones (Hsp27, PDIA3), RNA binding and transcription (hnRNPH3, ZNF511, ENO1, ATPA), unknown (ERp29, Galectin-3). Expressions of Hsp27 and Prx-2 in GCA specimens were further confirmed by immunohistochemical and western blot analysis. Conclusion: We identified 72 proteins medchemexpress of GCA, which may be helpful to construct the database and elucidate the molecular mechanisms of the carcinogenesis of GCA. Twenty three proteins regulated in IM may have a potential role in early detection targets of GCA. Key Word(s): 1. Gastric cardia tumor; 2. IM; 3. Biomarkers; 4. proteomics; Presenting Author: JINFENG DAI Additional Authors: LIJUN CAI, BIN LV Corresponding Author: LIJUN CAI Affiliations: The first affiliated hospital of Zhejiang university of TCM Objective: Given the high morbidity, postoperative recurrencerate and metastasis rate of gastric cancer, chemotherapy places an important role in its treatment. However, according to the data published by American Cancer Society, over 90% patients died more or less because of multiple drugs resistance (MDR).