Future experiments with a large sample size are needed to explore the usage of those minor alleles and to validate the predictive values of SNPs identified in this pilot study. Acknowledgements This work was supported by National Natural Science Foundation of PR China No. 30801384. The research was supported in part by the Intramural Research Program of the NIH, National Cancer Institute, check details Center for Cancer Research. Electronic supplementary material Additional file 1: Table S1: Statitical significance of the pairwise linkage disequilibrium analysis among SNP in mitochondrial D-loop. (DOC 28 KB) References 1. Gomaa AI, Khan SA, Toledano MB, Waked I, Taylor-Robinson SD: Hepatocellular carcinoma:
Epidemiology, risk factors and pathogenesis. World J Gastroenterol 2008, 14:4300–4308.selleck chemicals PubMedCrossRef 2. Sun Z, Ming L, Zhu X, Lu J: Prevention and control of hepatitis B in China. J Med Virol 2002, 67:447–450.PubMedCrossRef 3. Ferlay J, Bray F, Pisani P, Parkin DM: Globocan
2000: Cancer incidence, mortality and prevalence worldwide, version 1.0. In IARC Cancer Base No.5. Lyon, France: IARC Press; 2001. 4. Caldwell S, Park SH: The epidemiology of hepatocellular cancer: from the perspectives of public health problem to tumor biology. J Gastroenterol 2009, 44:96–101.PubMedCrossRef 5. Lu FM, Zhuang H: Management of hepatitis B in China. Chin Med J 2009, 122:3–4.PubMed Cilengitide clinical trial 6. Lu L, Wang X: Drug addition in China. Ann NY Acad Sci 2008, 1141:304–317.PubMedCrossRef 7. Schwarz KB: Oxidative stress during viral infection: a review. Free Radical Biol Med 1996, 21:641–649.CrossRef 8. Mansouri A, Fromenty B, Berson A, Robin MA, Grimbert S, Beaugrand M, Erlingr S, Pessayre D: Multiple hepatic mitochondrial DNA deletions suggest premature oxidative aging in alcoholic patients. J Hepatol 1997, 27:96–102.PubMedCrossRef 9. Shadel GS, Clayton DA: Mitochondrial DNA maintenance in vertebrates. Annu Rev Biochem Org 27569 1997, 66:409–435.PubMedCrossRef 10. DiMauro S, Schon EA: Mitochondrial DNA mutations in
human disease. Am J Med Genet 2001, 106:18–26.PubMedCrossRef 11. Beal MF: Mitochondia, free radicals, and neurodegeneration. Curr Opin Neurobiol 1996, 6:661–666.PubMedCrossRef 12. Lightowlers RN, Chinnery PF, Turnbull DM, Howell N: Mammalian mitochondrial genetics: heredity, heteroplasmy and disease. Trends Genet 1997, 13:450–455.PubMedCrossRef 13. Wallace DC: Mouse models for mitochondrial disease. Am J Med Genet 2001, 106:71–93.PubMedCrossRef 14. Fliss MS, Usadel H, Caballero OL, Wu L, Buta MR, Eleff SM, Jen J, Sidransky D: Facile detection of mitochondrial DNA mutations in tumors and bodily fluids. Science 2000, 287:2017–2019.PubMedCrossRef 15. Nomoto S, Yamashita K, Koshikawa K, Nakao A, Sidransky D: Mitochondrial D-loop mutation as clonal markers in multicentric hepatocellular carcimona and plasma. Clin Cancer Res 2002, 8:481–487.PubMed 16.