Observations of

a recent collection from Hedera in the UK confirmed that it is morphologically differ from D. helicis and D. pulla. The asexual morph produced by the isolate (M1078, in SMML click here culture collection, specimen BPI892914), from the UK has longer conidiophores (20–45 × 2–2.4 μm) and the paraphyses are abundant, while D. helicis and D. pulla have shorter conidiophores (8–15 × 1–2 μm) and paraphyses are absent. The ITS (KM111543) sequence similarity of the above referenced isolate from the UK confirmed that D. hederae can be a synonym of D. rudis (see Udayanga et al. (2014) for description and illustration). Type material of Diaporthe hederae examine UK, Boxhill, on vines of Hedera helix, July 1930, E.W. Mason Detr. L.E. Wehmeyer (BPI 1108438). Diaporthe neilliae Peck, Ann. Rep. N.Y. R428 purchase St. Mus. nat. Hist. 39: 52 (1887) [1886]. Fig. 8a–d Fig. 8 Morphology of Diaporthe neilliae (a–d) and D. pulla (e–g) a. Ectostoma on dead stem of Physocarpus opulifolius b–c. Asci d. Asci and ascospores e. Pycnidia on alfalfa stem on WA f. conidiophores g. α- conidia, Specimens: a–d. Holotype of D. neilliae BPI 616581, e-g.

selleck chemicals ex-epitype culture CBS 338.89, Scale bars: a = 2000 μm, b = 15 μm, c,d = 12 μm e = 1800 μm, f = 1 2 μm, g = 8 μm Perithecia on dead twigs, 200–300 μm diam, black, globose to conical, scattered irregularly, immersed in host tissue with elongated, 300–400 μm long necks protruding through substrata. Asci 36–50 μm × 7–10 μm (x̄±SD = 45 ± 5 × 8.5 ± 0.7, n = 30), unitunicate, 8-spored, sessile, elongate to clavate. Ascospores (11–)12–13.5(−14.5) × 3.5–4 μm (x̄±SD = 13 ± 0.8 × 3.8 ± 0.3, n = 30), hyaline, two-celled, often 4-guttulate, with larger guttules at centre and smaller one at ends, elongated to elliptical. Cultural characteristics: In dark at 25 °C for 1 wk, colonies on PDA slow growing,

2.6 ± 0.2 mm/day (n = 8), white, aerial mycelium, reverse white, turning to grey in centre; no conidia produced. Host range: On Glycogen branching enzyme Physocarpus opulifolius (Rosaceae). Geographic distribution: USA (New York). Type material: USA, New York, West Albany, on stems of Physocarpus opulifolius, C.H. Peck (NYS, holotype not examined, BPI 616581, isotype observed). Additional material examined USA, on Spiraea sp., September 1927, L.E. Wehmeyer (BPI 892921, CBS 144.27). Notes: Diaporthe neilliae is known only from the host species Physocarpus opulifolius; however, this host has been placed in various genera and has been reported as being on Neillia opulifolia, Opulaster opulifolus and Spiraea opulifolia, all names for the same species. This rosaceous host is native to North America, thus the isolate identified by L.E. Wehmeyer is used to represent this taxon; however, due to lack of information about its origin, it is not designated as the epitype. Diaporthe pulla Nitschke, Pyrenomycetes Germanici 2: 249 (1870) Fig. 8e–g = Phoma pulla Sacc., Michelia 2: 96 (1880) ≡ Phomopsis pulla (Sacc.) Traverso, Fl. ital. crypt.