In the BBH performed to identify common genes exclusive of pathogenic A1155463 bacteria, 851 clusters were obtained (Figure 2B). From these, 24 clusters involved in pathogenicity, protein secretion, and integration-recombination processes were selected, based on the best studied plant pathogen, Rhizobium tumefaciens C58 [27–29] in addition to clusters involved in biological nitrogen fixation.

R. tumefaciens was considered as the reference organism for pathogenesis because the symbionts in this study interact with plants and because in the animal pathogens of the Rhizobiales order, virulence-associated type IV secretion proteins homologous to R. tumefaciens Sepantronium price were identified [30–32]. Of the 24 clusters obtained, 11 of these clusters were analyzed in this study. The remaining 13 are related to protein secretion and integration-recombination (Figure 2B) (Table A2b of supplementary ICG-001 in vitro material in database). In the BBH performed with lower stringency for nitrogen-fixing

bacteria and bacteria involved in bioremediation, 41 extra clusters of interest were selected Fossariinae (Figure 2A, and Table A2a of supplementary material in database); however, they did not include all bacteria

used in the comparison. Among these clusters, two clusters were related to FixQ protein and two to NifS. Both FixQ and NifS clusters were composed by a separate group of bacteria. However, for each of these proteins, the clusters obtained were grouped in the analysis. Of the 41 clusters, 39 were analyzed. For pathogenic bacteria, of the clusters obtained in the analysis with lower stringency, 25 were obtained and 24 were selected for analysis (Figure 2B, and additional file 2) (in addition Table A2 of supplementary material in database). In the BBHs performed in this study, except for clusters related to protein secretion and integration-recombination, 96 clusters were selected. Of these, 81 are common or exclusive to nitrogen-fixing bacteria, bacteria involved in bioremediation, and pathogenic bacteria BBHs. Of these, 63 were of interest for analysis (except the clusters related to other evolutive mechanisms and those repeats for the same protein, which were considered as one) (Figure 2).