In Japan, Hirobe et al. Epacadostat (2005) had response from OPs at a rate of 20.4% when they made a survey on myocardial infarction morbidity of workers. When a questionnaires survey on OPs’ activities in SSEs was conducted, Terada et al. (2005) succeeded to obtain a higher response from OPs at 37.5% that was achieved when the survey was conducted in cooperation with medical associations in the regions. Muto et al. (1997) reported a similarly high response rate of 37.9% in a questionnaire

survey on the methods to persuade high management to support OHS, but the respondents included non-MDs (such as occupational nurses and safety and health supervisors) and OPs accounted for 37%. Taking these experiences by other study groups into consideration, the response rates in the present study may not be too low. The structure of the questionnaires used in the present study might have contributed to reduce response rates. The questionnaires set was rather bulky with 20 questions [including

some complicated ones (e.g., Q. 11, Q. 12 and Q. 13); see the appendix], and several questions (e.g., Q. 14 and Q. 15) requested answers in free writing. In fact, some OPs in both countries complained in the margin of the questionnaires sheet that “the questionnaire is too complicated and time consuming to complete”. The authors could not prepare a reward for the Z-VAD-FMK datasheet reply as well. These situations might have affected the response rate. There remain several points to be studied. The points include the satisfaction of employers and employees with current OHSs, effectiveness of OHSs to solve

or prevent problems, and possible effects of socio-economic Thiamine-diphosphate kinase factors. They are the subjects of future studies. In conclusion, the present survey suggests that service patterns are different between OPs in Japan and OPs in the Netherlands, i.e., more time for health and safety committees, worksite rounds, and overwork prevention in cases of Japanese OPs, whereas it is sick leave issues for OPs in the Netherlands. Both groups of OPs consider that the education of employers (possibly owner-managers in cases of SSEa) is important in addition to traditional education of workforces. These conclusions should, however, be taken as preliminary, due to various limitations especially low response rates. Further studies are apparently necessary before reaching solid conclusions. Acknowledgments We are grateful to the staff in the Coronel Institute of AMC and the Netherlands Society of Occupational Medicine, Mr. Jim de Beer and Miss Fumiko Ohashi who gave invaluable assistance for this study. Thanks are also due to National Federation of Industrial Health Organizations, Japan, Japan Society for Occupational Health, Society for the Study of Occupational Health Promotion, and staff in Kyoto Industrial Health Association, Japan.

Forestry 74:209–218CrossRef Gillman MP, Dodd ME (1998) The variability of orchid population size. Bot J Linn Soc 126:65–74CrossRef Gotelli Sorafenib order N, Ellison A (2004) A primer of ecological statistics. Sinauer Associates, Sunderland, p 510 Hegland SJ, van Leeuwen M, Oostermeijer JGB (2001) Population structure of Salvia pratensis in relation to vegetation and management of Dutch dry floodplain grasslands. J Appl Ecol 38:1277–1289CrossRef Horsley SB, Stout SL, de Calesta DS (2003) White-tailed deer impact on the vegetation dynamics of a northern hardwood forest. Ecol Appl 13:98–118CrossRef Hough AF (1965) A twenty-year record

of Understory vegetation change in a virgin Pennsylvania forest. Ecology 46:370–373CrossRef see more Hutchings MJ (1987) The population biology of the early spider orchid, Ophyrs sphegodes Mill. I. A demographic study from 1975 to 1984. J Ecol 75:711–727CrossRef Kauffman MJ, Frick WF, Linthicum J (2003) Estimator of habitat-specific demography and population

growth for Peregrine Falcons in California. Ecol Appl 13:1802–1816CrossRef Kery M, Gregg KB (2004) Demographic analysis of dormancy and survival in the terrestrial orchid Cypripedium reginae. J Ecol 92:686–695CrossRef Knight TM (2004) The effects of herbivory on pollen limitation on a declining population of Trillium grandiflorum. Ecol Appl 14:915–1928CrossRef Krueger LM, Peterson CJ (2006) Effects of white-tailed deer on Tsuga Canadensis regeneration: evidence of microsites as refugia from browsing. Am Midl Nat 156:353–362CrossRef Langdon K (1985) White-tailed deer Action Plan. On file at Catoctin Mountain Park, Thurmont. Supplement

to Natural Resource Management Plan, Catoctin Mountain Park, Catoctin Little RJA, Rubin DB (1987) Statistical analysis with missing data. Wiley, New York, p 408 Markus K, Grieser J, Beck C, Rudolf B, Franz R (2006) World Map of the Köppen–Geiger climate classification updated. Meteorologische Zeitschrift 15:259–263CrossRef Marquis DA (1981) Effect of Deer Browsing on Timber Production Cyclic nucleotide phosphodiesterase in Allegheny Hardwoods of Northwestern Pennsylvania. Northeastern Forest Experimental Station, U.S. Forest Service, Broomall, p 10 Maryland Department of Natural Resources (2013) Maryland Guide to Hunting and Trapping. Maryland Department of Natural Resources Wildlife and Heritage Service. http://​www.​eregulations.​com/​maryland/​hunting/​public-hunting-lands.  Accessed Dec 2013 Maryland Natural Heritage Program (2010) Rare, threatened and endangered plants of Maryland. Maryland Department of Natural Resources, Wildlife and Heritage Service, Annapolis McGraw JB, Furedi MA (2005) Deer browsing and population viability of a forest understory plant. Science 307:920–955PubMedCrossRef McShea WJ, Rappole JH (2000) Managing the abundance and diversity of breeding bird populations through manipulation of deer populations.

Although in another study [9] none of the isolates examined showed similarity with B. japonicum and B. liaoningense [9], sequence 146 in this study was closely related to B. japonicum USDA 38 (AF208514). Conclusion We have shown here that i) cowpea is strongly dependent on N2 fixation for its N nutrition in South Africa, Ghana and Botswana, ii) the diversity

of cowpea-nodulating bradyrhizobia was much higher in South Africa compared to Botswana and Ghana, iii) some strains from Southern Africa were phylogenetically very distinct, thus suggesting that they may be a new Bradyrhizobium species. Strain IGS type symbiotic efficiency was assessed for the first time in this study, and the data showed significant differences between and among the IGS types in terms

of their symbiotic efficiency. Acknowledgements This study was supported with funds from Tanespimycin concentration the McKnight Foundation to the South Africa Legumes Project, the National Research Foundation and the South African Research Chair in Agrochemurgy and Plant Symbioses to FDD, as well as a travel grant from the Organisation for the Prohibition of Chemical Weapons (OPCW) in The Netherlands to FPM. The NRF and TUT bursaries to FPM and AKB are also acknowledged. FPM is on study leave from the Botswana College of Agriculture (University of Botswana). References 1. Belane AK, Dakora FD: Measurement of N 2 fixation in 30 cowpea ( Vigna unguiculata L. Walp.) genotypes under field conditions in Ghana using 15 N natural abundance technique. Buparlisib cell line Symbiosis 2009, 48:47–57.CrossRef 2. Mpepereki S, Wollum AG, Makonese F: Diversity in symbiotic specificity of cowpea rhizobia indigenous to Zimbabwean soil. Plant Soil 1996, 186:167–171.CrossRef 3. Pule-Meulenberg F, Dakora FD: Assessing the symbiotic dependency of grain and tree legumes in N 2 fixation for their N nutrition in five agro-ecological zones of Botswana. Symbiosis 2009, 48:68–77.CrossRef 4. Naab JB, Chimphango SMB, Dakora FD: N 2 fixation in cowpea plants grown in farmers’ fields in the Upper Selleckchem Gemcitabine West Region of Ghana, measured using 15 N natural abundance. Symbiosis 2009, 48:37–46.CrossRef 5. Makoi JHJR, Chimphango SMB, Dakora FD: Effect of legume plant density

and mixed culture on symbiotic N 2 fixation in five cowpea ( Vigna unguiculata L. Walp.) genotypes in South Africa. Symbiosis 2009, 48:57–67.CrossRef 6. Law IJ, Botha WF, Majaule UC, Phalane FL: Symbiotic and genomic diversity of ‘cowpea’ bradyrhizobia from soils in Botswana and South Africa. Biol Fert Soils 2007, 43:653–663.CrossRef 7. Zhang WT, Yang JK, Yuan TY, Zhou JC: Genetic diversity and phylogeny of indigenous rhizobia from cowpea ( Vigna unguiculata (L.) Walp). 8. Steenkamp ET, Stepkowski T, Przymusiak A, Botha WJ, Law IJ: Cowpea and peanut in southern Africa are nodulated by diverse Bradyrhizobium strains harbouring genes that belong to the large pantropical clade common in Africa. Mol Phylogenet Evol 2008, 48:1131–1144.PubMedCrossRef 9.

At 24 and 72 h of cultivation, the expression of this gene was between 2 and 5 times higher in the 385-cyp61 hph /cyp61 zeo , CBS-cyp61 hph and Av2-cyp61 zeo strains than in the respective parental strains (Figure  8). Discussion Cytochrome P450 monooxygenases are involved in the oxidative metabolism of an enormous diversity of substrates, taking part in primary, secondary and xenobiotic metabolism. CYP51 and CYP61 are structurally and functionally conserved fungal P450s involved VX-770 mouse in membrane ergosterol biosynthesis [36], and the role

of CYP61 as a C22-desaturase in fungal membrane sterol synthesis has been elucidated in S. cerevisiae[24] and Candida glabrata[37]. In this study, we isolated and characterized a gene, CYP61, from X. dendrorhous that has nine exons, encodes a putative 526-residue polypeptide and shares significant similitude and identity with the C22-sterol desaturase from S. cerevisiae[25]. We could predict several P450 characteristic secondary structural elements, www.selleckchem.com/products/3-methyladenine.html and we identified three residues in CYP61 that are completely conserved in P450s. Together, these observations support the hypothesis that the X. dendrohous CYP61 gene encodes the cytochrome P450 CYP61. As in other organisms [25], the CYP61 gene is not essential

for the X. dendrorhous viability, even though we demonstrated that it is involved in ergosterol biosynthesis. Disruption of the CYP61 gene prevents ergosterol biosynthesis and leads to the accumulation

of other intermediary sterols including ergosta-5,8-dien-3-ol and ergosta-5,8,22-trien-3-ol. Contrary to our findings, the specific mutation of ERG5 in S. cerevisiae results in the predominant accumulation of ergosta-5,7-dien-3-ol, although the C22-desaturase substrate is ergosta-5,7,24-trien-3-ol [25, 38]. Like in X. dendrohous, ergosta-5,8,22-trien-3-ol accumulation has been observed in other fungi, such as C. neoformans, after the inhibition of the ERG6-encoding enzyme [39] and in nystatin-resistant Neurospora crassa strains that are unable to produce ergosterol [40]. Although our second found intermediary, ergosta-5,8-dien-3-ol, is an atypical sterol, it has Succinyl-CoA been detected in fungi strains that are unable to synthetize ergosterol that in turn are resistant to fungicidal polyenes, such as nystatin and primaricin; polyenes bind ergosterol in the fungal cell membrane, creating channels that disrupt the transmembrane potential and its functions [41]. This phenomenon was observed in a nystatin-resistant S. cerevisiae strain [42] and primaricin-resistant Aspergillus nidulans strains [43]. Clearly, these observations and our results indicate the existence of alternative sterol biosynthesis pathways, which require further studies.

Table 2 Diagnostic accuracy of physical examination, transvaginal ultrasonography,

and both for diagnosing surgical emergencies   Physical examination alone TVUS alone Strategy combining physical examination andTVUS† Se% (n/N) [95% CI] Sp% (n/N) [95% CI] LR + LR – Se (n/N) [95% CI] Sp (n/N) [95% CI] LR+ Dabrafenib LR – Se (n/N) [95% CI] Sp (n/N) [95% CI] LR+ LR – Overall population 87% (121/139) [82–93] 33% (31/95) [23–42] 1.3 0.4 94% (131/139) [90–98] 27% (26/95) [18–36] 1.3 0.2 99% (138/139) [98–100] 7% (7/95) [2–13] 1.1 0.1 Pregnant women 84% (81/97) [76–91] 42% (22/53) [28–55] 1.4 0.4 96% (93/97) [92–100] 13% (7/53) [4–22] 1.1 0.3 99% (96/97) [97–100] 6% (3/53) [0–12] 1.1 0.2 Non-pregnant women 95% (40/42) [89–100] 21% (9/42) [19–34] 1.2 0.2 91% (38/42) [82–99] 45% (19/42) [30–60] 1.6 0.2 100% (42/42) [92 – 100] 10% (4/42) [1–18] 1.1 0 Se, sensitivity; CI, confidence interval; Sp, specificity; LR, likelihood ratio. †Corresponds to a strategy of routine TVUS regardless of the clinical findings, abnormal findings include abnormal examination OR abnormal TVUS. TVUS, transvaginal ultrasonography; Se, sensitivity; Sp, specificity;

LR+, positive likelihood ratio; LR-, negative likelihood ratio; 95%CI, 95 % confidence interval. Table 3 Diagnoses in patients with a laparoscopy diagnosis of surgical emergency PI3K Inhibitor Library price but had negative physical examination or negative transvaginal ultrasonography or negative with both examinations combined   FN, physical examination, n (%) FN, TVUS, n (%)

FN, physical examination combined with TVUS†, n (%) Total number of patients with surgical emergencies, N Ectopic pregnancy 14 (15%) 1 (1%) 0 91 Pelvic peritonitis 0 1 (4 %) 0 25 Adnexal torsion 3 (20%) 3 (20%) 1 (7%) 15 Appendicitis 0 1 (25%) 0 4 Intestinal obstruction 0 2 (100%) 0 2 Ruptured hemorrhagic cyst 1 (50%) 0 0 2 Total 18 (13%) 8 (6%) 1 (0.7%) 139 Percentages were computed by dividing the number of false negatives by the total number of surgical emergencies. FN, False negatives; TVUS, transvaginal ultrasonography. †Corresponds to a strategy of routine TVUS regardless of the clinical findings, abnormal findings include abnormal examination OR abnormal TVUS. The strategy combining physical examination and TVUS in first-line was better than the strategy including only physical examination acetylcholine according to our criteria in which surgical emergencies were suspected based on abnormal clinical OR TVUS findings. This strategy decreased the false-negative rate from 13% (physical examination alone) to less than 1% (Table  3). The strategy combining physical examination and TVUS was the one maximizing Se and decreased negative LR to an acceptable rate of 0.1. When pregnant and nonpregnant patients were analyzed separately, the results were unchanged (Table  2). Discussion According to our data, physical examination cannot be used alone to safely rule out a surgical emergency in a woman presenting with acute pelvic pain.

In each case, complementation was observed (Fig. 3). Thus, at least for this selection of genes www.selleckchem.com/products/ABT-263.html it is likely that the gene products contributed to reducing the lethal effects of nalidixic acid. While these data do not assure that

complementation will occur in the other cases, they give us confidence to move forward with the study of the bacterial response to lethal stress. We note in some cases paradoxical survival occurred at high concentrations of nalidixic acid. This phenomenon, which is unexplained, is commonly observed with quinolones [39]. Figure 3 Complementation of hyperlethal phenotype by cloned genes. Plasmids containing wild-type genes were transformed into the corresponding Tn5-containing mutants. The strains harboring the plasmids were then tested for nalidixic acid-mediated lethality by treating mid-log phase cells with various concentrations of nalidixic acid for 2 hr at 37°C. Percent of control indicates percent survival of treated cells relative to untreated cells sampled at the time of drug addition. For ycjW, yrbB, and ybcM, the expression was induced by adding 1 mM of IPTG 2 hr before nalidixic acid treatment. Similar results were obtained in a replicate experiment. Conclusions The present work described a novel screening process for identifying genes involved in protecting E. coli from quinolone-mediated death due to events occurring after formation of selleckchem quinolone-gyrase-DNA

complexes. Using this screen we identified 14 poorly characterized genes. Scattered evidence suggests that many of these Buspirone HCl genes are linked to protective stress responses, which is supported by our finding that mutations in these putative protective genes resulted in decreased survival following treatment with several stressors. The diverse set of genes described may serve as potential targets

for future screening of small-molecule antimicrobial potentiators. Acknowledgements This work was supported by National Natural Science Foundation of China (Grant No. 30860012) and Natural Science Foundation of Yunnan Province of China (Grant No. 2005C0007R) to T.L, NIH grants AI35257 and AI 073491 to K.D, and NIH grant AI068014 to XZ. References 1. Levy SB: Antibiotic resistance-the problem intensifies. Adv Drug Deliv Rev 2005,57(10):1446–1450.PubMedCrossRef 2. Levy SB, Marshall B: Antibacterial resistance worldwide: causes, challenges and responses. Nat Med 2004,10(12 Suppl):S122–129.PubMedCrossRef 3. Buynak JD: Understanding the longevity of the beta-lactam antibiotics and of antibiotic/beta-lactamase inhibitor combinations. Biochem Pharmacol 2006,71(7):930–940.PubMedCrossRef 4. Nelson ML, Levy SB: Reversal of tetracycline resistance mediated by different bacterial tetracycline resistance determinants by an inhibitor of the Tet(B) antiport protein. Antimicrobial agents and chemotherapy 1999,43(7):1719–1724.PubMed 5.

Figure 3 Comparison of genetic determinants

of chromate resistance in other bacterial strains versus Protein Tyrosine Kinase inhibitor B. cereus SJ1. (a) Genetic context of the chromate operon chrIA and arsenic resistance operon arsRBCDA in B. cereus SJ1. (b) Genetic context of the chromate operon chrIA1 in B. thuringiensis serovar konkukian str. 97-27. B. thuringiensis str. 97-27 [GenBank: AE017355]; B. anthracis str. Ames Ancestor [GenBank: AE017334]; B. anthracis str. Ames [GenBank: NC003997]; B. anthracis str. Sterne [GenBank: AE017225]; B. cereus E33L [GenBank: CP000001]; B. thuringiensis str. Al Hakam [GenBank: NC008600] and B. cereus ATCC 10987 [GenBank: AE017194]. Heavy metal tolerance of B. selleck screening library cereus SJ1 and putative genes responsible for heavy metal resistance Since B. cereus SJ1 was isolated from industrial wastewater containing various toxic elements in addition to chromium, the MICs of B. cereus SJ1 for these heavy metals were determined. For B. cereus SJ1, the highest resistance was found for As(V), while Hg(II) was the most toxic compared

to the other metal ions. When B. cereus SJ1 was incubated with increasing As concentration, no viable cells were recovered at concentrations above 50 mM As(V) and 4 mM As(III). The MICs of B. cereus SJ1 for Cu(II), Co(II), Ni(II), Cd(II), Ag(I) and Hg(II) were 0.9 mM, 0.8 mM, 0.7 mM, 0.2 mM, 0.02 mM and 0.007 mM, respectively. In order to survive in such unfavorable habitat, B. cereus SJ1 must have various determinants to tolerate such harsh conditions. For example, the copper concentration of the wastewater was as high as 0.65 mM and the MIC of B. cereus SJ1 to copper was 0.9 mM in R2A medium. When we analyzed the genome sequence of B. cereus SJ1, several genes related to copper resistance including copper-exporting P-type ATPase CopA, copper export protein CopC, copper resistance protein CopD, copper homeostasis protein CutC and two multicopper oxidases were identified. Furthermore, many other putative heptaminol heavy metal resistance

genes including those for As, Zn, Mn, Co, Cd, Te and Hg were also identified in the B. cereus SJ1 draft genome (Additional file 2). Chromate reduction is constitutive The difference in chromate reducing ability of B. cereus SJ1 with and without Cr(VI) induction was not significant (Figure 4A). Although less rapid chromate reduction was observed in B. cereus SJ1 cells induced before inoculation during the first 32 h, both cultures emerged at approximately 85% chromate reduced within 55 h. No abiotic Cr(VI) reduction was observed in LB medium without bacterial inoculation. Induction of genes possibly responsible for chromate reduction was also evaluated by RT-PCR. As shown in Figure 5, all the four nitR genes and the azoR gene were expressed constitutively.

The 3’ end of the insert (module E) is homologous to Tn1806

of S. pneumoniae which confers erythromycin resistance. Although this element has not been shown to transfer via conjugation, transfer via transformation was shown [22]. In C. difficile strain M120 this element appears to be the backbone into which several other elements have been inserted (see Figure 1 top panel). The first 7.3 kb on the 5’ end of the insert (module A) has only moderate homology (60–70% maximum sequence identity) to known sequences. Interestingly, this part of the insert contains 2 putative modification DNA methylases and a putative endonuclease, possibly enabling a form of molecular vaccination as described by Kobayashi et al. [23]. During this process methylation protects the incoming

GW-572016 manufacturer element from host endonucleases and, following integration, will protect the host chromosome from endonucleases present on other mobile genetic elements. This sequence is followed by a complete prophage of approximately 39.5 kb (module B), which shows 92% sequence identity to a Thermoanaerobacter sp. prophage (Genbank accession no. CP002210). The next 4.5 kb stretch (module C) is 99% identical to part of the Enterococcus faecalis plasmid pEF418 containing, amongst others, a putative methyltransferase and a putative spectinomycin adenyltransferase (ant(9)Ia) [24]. It is also described to be part of a pathogenicity island in Streptococcus suis[25]. Finally, an insertion of approximately Stem Cell Compound Library cost 4.5 kb (module D) with 90% sequence identity to the transferable pathogenicity island of Campylobacter fetus subsp fetus[26] is present within the sequence of Tn1806. This sequence contains, amongst others, putative tet(44) and ant(6)-Ib genes, which could respectively confer tetracycline and streptomycin resistance. The G + C content of the entire insert (34%) was significantly higher than that of the IKBKE entire genome (29%), clearly indicating that the insert was of foreign origin (see Additional file 1). In addition, within the insert the different modules could be distinguished by their G + C contents. The G + C content

of module A, B, C, D and E was 31%, 41%, 35%, 28% and 31%, respectively. The 100 kb insert is a transposon Based on the bioinformatic comparison of the insert described above, the possible excision of 3 (independent) elements was predicted. Primers were designed (primers 14–20, see Table 3) to amplify the circular intermediates of the complete insert (primers 14 and 15), the putative Thermoanaerobacter sp. phage (module B, primers 15 and 16) and the C. fetus pathogenicity island (module D, primers 17 and 18) of the element. PCR confirmed only the excision and circularisation of the entire insert (results not shown). It is expected that the serine recombinase at the 3’ end of the element is responsible for excision (see Table 1).

Front Biosci 2002, 7:d1798–1814.CrossRefPubMed 10. Pozzi G, Masala L, Iannelli F, Manganelli R, Havarstein LS, Piccoli L, Simon D, Morrison DA: Competence for genetic transformation in encapsulated strains of Streptococcus pneumoniae : two allelic variants of the peptide pheromone. J Bacteriol 1996, 178:6087–6090.PubMed 11. Ramirez M,

Morrison DA, Tomasz A: Ubiquitous distribution of the competence related genes comA and comC among isolates of Streptococcus pneumoniae. Microb Drug Resist 1997, 3:39–52.CrossRefPubMed 12. Whatmore AM, Barcus VA, Dowson CG: Genetic diversity of the streptococcal competence ( com ) gene locus. J Bacteriol 1999, 181:3144–3154.PubMed 13. Guiral S, Mitchell TJ, Martin B, Claverys JP: Competence-programmed predation of noncompetent cells find more in the human pathogen Streptococcus pneumoniae : genetic requirements. Proc Natl Acad Sci USA 2005, 102:8710–8715.CrossRefPubMed

14. Claverys JP, Martin B, Havarstein LS: Competence-induced fratricide in streptococci. Mol Microbiol 2007, 64:1423–1433.CrossRefPubMed 15. Claverys JP, Havarstein LS: Cannibalism and fratricide: mechanisms and raisons d’être. Nat Rev Microbiol 2007, 5:219–229.CrossRefPubMed 16. Gilmore MS, Haas W: The selective advantage of microbial fratricide. Proc Natl Acad Sci USA 2005, 102:8401–8402.CrossRefPubMed 17. Havarstein LS, Martin B, Johnsborg O, Granadel C, Claverys JP: New insights into the pneumococcal fratricide: relationship to clumping and identification of a novel immunity factor. Mol Microbiol 2006, 59:1297–1307.CrossRefPubMed 18. Gray BM, Converse GM 3rd, Dillon HC Jr: Epidemiologic studies of Streptococcus pneumoniae in infants: MI-503 order acquisition, carriage, and infection during the first 24 months of life. J Infect Dis 1980, 142:923–933.PubMed 19. Brugger SD, Hathaway LJ, of Muhlemann K: Detection of Streptococcus pneumoniae strain cocolonization in the nasopharynx. J Clin Microbiol 2009, 47:1750–1756.CrossRefPubMed 20. Havarstein LS, Hakenbeck R, Gaustad P: Natural competence in the genus Streptococcus : evidence

that streptococci can change pherotype by interspecies recombinational exchanges. J Bacteriol 1997, 179:6589–6594.PubMed 21. Tortosa P, Dubnau D: Competence for transformation: a matter of taste. Curr Opin Microbiol 1999, 2:588–592.CrossRefPubMed 22. Claverys JP, Prudhomme M, Martin B: Induction of competence regulons as a general response to stress in Gram-positive bacteria. Annu Rev Microbiol 2006, 60:451–475.CrossRefPubMed 23. Park IH, Pritchard DG, Cartee R, Brandao A, Brandileone MC, Nahm MH: Discovery of a new capsular serotype (6C) within serogroup 6 of Streptococcus pneumoniae. J Clin Microbiol 2007, 45:1225–1233.CrossRefPubMed 24. Hausdorff WP, Feikin DR, Klugman KP: Epidemiological differences among pneumococcal serotypes. Lancet Infect Dis 2005, 5:83–93.PubMed 25. Aguiar SI, Serrano I, Pinto FR, Melo-Cristino J, Ramirez M: The presence of the pilus locus is a clonal property among pneumococcal invasive isolates.

Plant Physiol Biochem 36:407–417. doi:10.​1016/​S0981-9428(98)80204-0 CrossRef Harwood JL (1998) Involvement of chloroplast lipids in the reaction of plants submitted Fluorouracil to stress. In: Siegenthaler PA, Murata N (eds) Advances in photosynthesis. Lipids in photosynthesis. Kluwer, Dordrecht, pp 287–302 Hendrickson L, Vlčkova A, Selstam E, Huner N, Öquist G, Hurry V (2006) Cold acclimation of the Arabidopsis dgd1 mutant results in recovery from photosystem I-limited photosynthesis. FEBS Lett 580:4959–4968. doi:10.​1016/​j.​febslet.​2006.​07.​081 CrossRefPubMed Ihalainen JA, Jensen PE, Haldrup A, van Stokkum IHM, van Grondelle R, Scheller HV, Dekker JP (2002)

Pigment organization and energy transfer dynamics in isolated photosystem I (PSI) complexes from Arabidopsis thaliana EGFR phosphorylation depleted of the PSI-G, PSI-K, PSI-L, or PSI-N subunit. Biophys J 83:2190–2201. doi:10.​1016/​S0006-3495(02)73979-9 CrossRefPubMed Ihalainen JA, van Stokkum IHM, Gibasiewicz K, Germano M, van Grondelle R, Dekker JP (2005) Kinetics of excitation trapping in intact photosystem I of Chlamydomonas reinhardtii and Arabidopsis thaliana. Biochim Biophys Acta 1706:267–275. doi:10.​1016/​j.​bbabio.​2004.​11.​007 CrossRefPubMed Ivanov AG, Hendrickson L, Krol M, Selstam E, Öquist G, Hurry V, Huner NPA (2006) Digalactosyl-diacylglycerol deficiency impairs the capacity for photosynthetic

intersystem electron transport and state transitions in Arabidopsis thaliana due to photosystem I acceptor-side limitations. Plant Cell Physiol 47:1146–1157. doi:10.​1093/​pcp/​pcj089 CrossRefPubMed Jarvis P, Dörmann P, Peto CA, Lutes J, Benning C, Chory J (2000) Galactolipid deficiency and abnormal chloroplast development in the Arabidopsis MGD synthase 1 mutant. Proc Natl Acad Sci USA 97:8175–8179. doi:10.​1073/​pnas.​100132197 CrossRefPubMed Junge W (1977) Membrane potentials in photosynthesis. Annu Rev Plant Physiol 128:503–536. doi:10.​1146/​annurev.​pp.​28.​060177.​002443 CrossRef Kovács L, Damkjar J, Kereiche S, Ilioaia C, Ruban AV, Boekema EJ,

Jansson S, Horton P (2006) Lack of the light-harvesting www.selleck.co.jp/products/Staurosporine.html complex CP24 affects the structure and function of the grana membranes of higher plant chloroplasts. Plant Cell 18:3106–3120. doi:10.​1105/​tpc.​106.​045641 CrossRefPubMed Krumova SB, Koehorst RBM, Bóta A, Páli T, van Hoek A, Garab G, van Amerongen H (2008a) Temperature dependence of the lipid packing in thylakoid membranes studied by time- and spectrally resolved fluorescence of Merocyanine 540. Biochim Biophys Acta 1778:2823–2833. doi:#10.​1016/​j.​bbamem.​2008.​09.​007 CrossRefPubMed Krumova SB, Dijkema C, de Waard P, Van As H, Garab G, van Amerongen H (2008b) Phase behavior of phosphatidylglycerol in spinach thylakoid membranes as revealed by 31P-NMR.