09; r=-0.166, p=0.325; r=-0.208, p=0.217; respectively). In PD patients, salivary concentrations of sodium, potassium, chloride were
higher but amylase was lower than in controls (p=0.02, p<0.001, p=0.003, p=0.04, respectively). When mild and severe PD patients were compared there were no significant difference between amylase, potassium, and chloride concentrations of the groups (p=0.07, p=0.32, p=0.16, respectively).\n\nConclusions: PD is associated with decreased salivary production, abnormally high electrolyte and low amylase concentrations. Drooling of saliva is caused by concomitant swallowing difficulties. Thus, using botulinum toxin or anticolinergic drugs in treatment of drooling may cause xerostomia.”
“Diabetes is characterized by high blood glucose level find protocol due to either autoimmune destruction
of islet beta-cells or insufficient insulin secretion or glucose non-responsive production of insulin by beta-cells. It is highly desired to Liproxstatin-1 replace biological functional beta-cells for the treatment of diabetes. Unfortunately, beta-cells proliferate with an extremely low rate. This cellular property hinders cell-based therapy for clinical application. Many attempts have been made to develop techniques that allow production of large quantities of clinically relevant islet beta-cells in vitro. A line of studies evidently demonstrate that beta-cells can proliferate under certain circumstances, giving the hopes for generating and expanding these cells in vitro and transplanting them to the recipient. In this review, we discuss the requirements of microenvironmental stimuli that stimulate beta-cell proliferation in cell cultures. We highlight advanced approaches for augmentation of beta-cell expansion that have recently emerged in this field. Furthermore, knowing the signaling pathways and molecular mechanisms would enable manipulating cell proliferation and optimizing its insulin secretory function. Thus, signaling pathways involved in the enhancement of cell proliferation are discussed as well.”
“Background: The preservation of hard and soft tissue volume, partially lost after tooth removal, can potentially reduce the need for the more demanding
augmentation procedures used in implant-supported rehabilitation. Baf-A1 cost The objective of this research study is to investigate the effect of filling with xenogeneic material the postextractive sockets of two surgical procedures (flapless versus flapped). Methods: In this prospective randomized clinical survey, two types of socket preservation were performed on two groups of patients: the control, treated via full-thickness mucoperiosteal flap, and the test, via a flapless procedure. Anatomic measurements and related outcome variables at the third month were analyzed using multiway analysis of variance. Multiple comparison tests, using Tukey honestly significant difference test, and appropriate pairwise comparison tests for independent samples were carried out.
Sequencing results, restriction fragment length polymorphism (RFLP) and phylogenetic analysis of sequences from the 1248-bp R16F2n/R16R2 products and partial ribosomal protein operon genes revealed the FcD-YT phytoplasma as a member of subgroup 16SrV-B, rpV-C. Two RFLP patterns were observed in recombinant colonies from the cloned 16S rDNA gene with endonucleases HpaII and MseI. To our knowledge, this is the first report of 16SrV group phytoplasma declining flowering cherry trees in China.”
aim of https://www.selleckchem.com/products/lazertinib-yh25448-gns-1480.html this study was to evaluate the cleaning effectiveness of implant prophylaxis instruments on polished and acid-etched implant surfaces. Materials and Methods: Biofilm layers of Streptococcus mutans were grown on a total of 80 titanium disks; 40 disks were polished and 40 were acid-etched. Five disks of each surface were cleaned using each of seven implant prophylaxis instruments: (1) manual plastic curette, (2) manual carbon fiber-reinforced plastic (CFRP) curette, (3) sonic-driven prophylaxis brush, (4) rotating rubber cup with prophylaxis paste, (5) sonic-driven polyether ether ketone (PEEK) plastic tip, (6) ultrasonic-driven
PEEK plastic tip, and (7) air polishing with amino acid (glycine) powder. Ten disks (five of each surface type) served as controls. Torin 2 After cleaning, the surfaces with remaining bacteria were assessed by light microscopy. Statistical analyses of the results were performed with one-way and two-way analyses of variance with Bonferroni-Dunn
multiple comparisons post hoc analysis (alpha Buparlisib in vivo = .05). Results: The cleaning effectiveness of the plastic curette was significantly lower than those of all machine-driven instruments on the polished surface. Significantly lower cleaning effectiveness occurred with the CFRP curette compared to the prophylaxis brush and to both oscillating PEEK plastic tips on the polished surface. The rubber cup provided less cleaning effectiveness compared to the ultrasonic PEEK plastic tip and air polishing on the acid-etched surface. Superior results, with less than 4% of the biofilm remaining, were obtained for both oscillating PEEK plastic tips and air polishing on both implant surfaces. The cleaning ability of the prophylaxis brush, rubber cup, and ultrasonic PEEK plastic tip differed significantly between both surface structures. Conclusions: Cleaning effectiveness, ie, less than 4% of the biofilm remaining, was not observed with all tested implant prophylaxis instruments. The cleaning ability of the devices depended on the implant surface structure.”
“Since 2009 pandemic, international health authorities recommended monitoring severe and complicated cases of respiratory disease, that is, severe acute respiratory infection (SARI) and acute respiratory distress syndrome (ARDS).
The relative weights and the scores from the NRS were used to compute the PACADI score (range 0 to 10). The patients also completed Edmonton Symptom Assessment
System (ESAS) and EQ-5D.\n\nDimensions reported by more than 20 % of the patients were included in the PACADI score (relative weights in parenthesis): pain/discomfort (0.16), fatigue (0.16), anxiety (0.15), bowel/digestive NCT-501 in vivo problems (0.14), loss of appetite (0.13), dry mouth (0.11), itchiness (0.08), and nausea (0.07). The PACADI score in the 80 PC patients had a mean (SD) value of 3.26 (2.06) (95 % CI 2.80, 3.71), was moderately to strongly correlated to ESAS sense of well-being (r = 0.69) and EQ-5D (r = -0.52), and discriminated significantly between patients with and without PC.\n\nThe PACADI score is a new eight-item, patient-derived, disease-specific measure. Preliminary validation regarding construct validity and discrimination encourages further validation in independent patient samples.”
“Background: We have recently shown that intranasal administration of mouse [D-Leu-4]-OB3 reconstituted in Intravail (R) to male Swiss Webster mice resulted in significantly higher bioavailability than commonly used injections methods of delivery. The absorption pro. le associated with intranasal
delivery of mouse [D-Leu-4]-OB3 showed an early peak representing absorption across the nasal mucosa, and a later peak suggesting selleck chemicals llc a gastrointestinal site of uptake.\n\nAim and Methods: In the present study, we examined the effects of orally administered (by gavage) mouse [d-Leu-4]-OB3 on energy balance, glycaemic control and serum osteocalcin levels
in male C57BL/6J wild-type and ob/ob mice allowed food and water ad libitum or calorie restricted by 40% of normal intake.\n\nResults: In wild-type mice fed ad libitum, oral delivery of mouse [d-Leu-4]-OB3 reduced body weight gain, food intake and serum glucose, by 4.4, 6.8 and 28.2% respectively. Serum osteocalcin levels and water intake were essentially 3-Methyladenine chemical structure the same in control and treated wild-type mice. In ob/ob mice fed ad libitum, mouse [d-Leu-4]-OB3 reduced body weight gain, food intake, water intake and serum glucose by 11.6, 16.5, 22.4 and 24.4% respectively. Serum osteocalcin in ob/ob mice treated with mouse [d-Leu-4]-OB3 was elevated by 62% over controls. Calorie restriction alone caused significant weight loss in both wild-type (9.0%) and ob/ob (4.8%) mice, and mouse [d-Leu-4]-OB3 did not further enhance this weight loss. As expected, serum glucose levels in wild-type and ob/ob mice were significantly reduced by calorie restriction alone. Mouse [d-Leu-4]-OB3 further reduced serum glucose in wild-type mice and normalized levels in ob/ob mice. Calorie restriction alone reduced serum osteocalcin levels by 44.2% in wild-type mice and by 19.1% in ob/ob mice. Mouse [d-Leu-4]-OB3 prevented this decrease in groups of mice.
Abnormal expression and/or dysfunction of NEK8 are related to cancer development and progression. However, the mechanisms that regulate NEK8 are not well declared. We demonstrated here that pVHL may be involved in regulating NEK8. We found that CAK-I cells with wild-type
vhl expressed a lower level of NEK8 than the cells loss of vhl, such as 786-O, 769-P, and A-498 cells. Moreover, pVHL overexpression down-regulated the NEK8 protein in 786-O cells, whereas pVHL knockdown up-regulated NEK8 in CAK-I cells. In addition, we found that the positive hypoxia response elements (HREs) are located in the promoter of the nek8 sequence and hypoxia Z-VAD-FMK concentration could induce nek8 expression in different cell types. Consistent with this, down-regulation of hypoxia-inducible factors alpha (HIF-1 alpha or HIF-2 alpha) by isoform-specific siRNA reduced the ability selleck chemicals of hypoxia inducing nek8 expression. In vivo, NEK8 and HIF-1 alpha expression were increased in kidneys of rats subjected to an experimental hypoxia model of ischemia and reperfusion. Furthermore, NEK8 siRNA transfection significantly blocked pVHL-knockdown-induced cilia disassembling, through impairing the pVHL-knockdown-up-regulated NEK8 expression. These results support that nek8 may be a novel hypoxia-inducible gene. In conclusion, our findings show that nek8 may be a new HIF target gene and pVHL can down-regulate NEK8 via HIFs to maintain the
primary cilia structure in human renal cancer cells.”
“Plant growth and defence are both fuelled by compounds synthesized from a common pool of carbon and nitrogen, implying the existence
of a competition for carbon and nitrogen allocation to both metabolisms.\n\nThe ratio of carbon to nitrogen (C:N) of an organ is often regarded as a convenient indicator of growth and quality. The purpose of this work was to assess whether or not it is possible to extend its use to characterize the trade-off between growth and defence processes. Therefore, we calculated C:N ratios in the pool of resources and in the total plant, and Vorinostat correlated them to the concentrations of diverse compounds of the primary and secondary metabolisms in young tomatoes.\n\nPlants were grown hydroponically at N availabilities either limiting (0.1 mM) or not (7 mM) for growth in two glasshouses maintained either under ambient or enriched (700 vpm) air CO2. These conditions yielded a large array of C:N in fully developed leaves, developing leaves, stem and roots, sampled 27, 35 and 47 days after sowing. Growth parameters and tissue concentrations of primary metabolites (carbohydrates, starch), defence-related compounds (polyphenols, glycoalkaloids), lignin, nitrate, ammonium, C and N were analyzed. Net CO2 exchange rate was also measured at the last sampling date.\n\nLow N limited plant growth more than photosynthesis. The C:N in the resource pool was far higher than the total C:N.
An urgent cesarean section was performed under spinal anesthesia with the chest drain in see more situ. During her follow-up the thoracic computed tomography revealed a bulla at the lower lobe of the right hemithorax. We performed right thoracotomy and bullectomy.”
“A numerical study has been executed to analyze the effects of Reynolds and Prandtl number on mixed convective flow and heat transfer characteristics inside a ventilated cavity in presence of a heat-generating solid circular obstacle placed at the center. The inlet opening is at the bottom of the left wall, while the outlet one is at the top of the right wall and all the walls of the cavity are considered to be adiabatic. Galerkin weighted residual
finite element method is used to solve the governing equations of mass, momentum and energy. Results are presented in terms of streamlines, isotherms, the average Nusselt number, the Drag force and the average fluid temperature in the cavity for different combinations of controlling parameters namely, Reynolds number, Prandtl number and Richardson number. The results indicate that
the flow and thermal fields as well as the heat transfer rate, the Drag force and the average fluid temperature in the cavity depend significantly on the mentioned parameters. (C) 2011 Elsevier Inc. All rights reserved.”
“The reactivity of human fetoplacental arteries is regulated by humoral and local factors of maternal and fetal origin. The chorioallantoic (CA) arteries buy Sapanisertib of bird embryos are homologous to fetoplacental arteries and fulfill
the same gas-exchange purpose without maternal influences, but their reactivity has not been studied in detail. In the present study we hypothesized that CA arteries would respond to vasoactive factors similarly to fetoplacental arteries and the response would change during development between maximal vascular CA expansion (15 of the 21 days incubation period) and prior to hatching. Therefore, we analyzed the reactivity of third order arteries (similar to 200 mu m) from the CA membrane of 15 and 19 day chicken embryos. CA arteries contracted in response to K(+), the thromboxane A(2) mimetic U46619, endothelin-1, acetylcholine and acute hypoxia, but showed no reaction to alpha-adrenergic stimulation (phenylephrine). The nitric oxide donor sodium nitroprusside, the adenylyl cyclase MK-2206 ic50 agonist forskolin, and the beta-adrenergic agonist isoproterenol relaxed CA arteries precontracted with K(+) or U46619. The contraction evoked by acetylcholine and the relaxations evoked by sodium nitroprusside and isoproterenol decreased with incubation age. In conclusion, CA arteries share many characteristics with human fetoplacental arteries, such as pronounced relaxation to beta-adrenergic stimuli and hypoxic vasoconstriction. Our study will be the foundation for future studies to explain disparate and common responses of the CA and fetoplacental vasculature.
The exponential parameters of the Gaussians are variationally optimized with the aid of the analytical energy gradient determined with respect to those parameters. The calculated state energies are compared with the available experimental data. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.3698584]“
“Purpose: To determine the rates of globe-sparing treatment and useful final visual function in patients with primary lacrimal sac/nasolacrimal duct carcinomas treated with multidisciplinary therapy.\n\nMethods: The medical records of 14 patients with primary lacrimal sac/nasolacrimal duct carcinoma treated at 1 institution were retrospectively reviewed.\n\nResults:
The patients were 9 men and 5 women; the median age at diagnosis was 58.5 years (range, 45-73 years). Seven patients presented with epiphora, 7 with a palpable this website mass in the inferomedial orbit, and 2 with dacryocystitis. In 3 patients, the diagnosis of cancer was not considered
until during or after dacryocystorhinostomy. Seven patients had squamous cell carcinoma, 2 transitional cell carcinoma, 2 adenoid cystic carcinoma, and 1 each adenocarcinoma, poorly differentiated carcinoma, and inverted papilloma with carcinoma in situ transformation. Nine ASP2215 supplier patients underwent surgical resection of the lacrimal sac and nasolacrimal duct and resection of the medial upper and lower eyelids, including canaliculi, partial ethmoidectomy, and medial maxillectomy. One patient underwent lacrimal sac biopsy only as another primary malignancy was S63845 discovered during the work-up for systemic disease. Four patients underwent orbital exenteration because of extensive involvement of the orbital soft tissue. Radiotherapy was recommended for 13 patients; in 1 patient, radiotherapy was not recommended because the patient had an inverted papilloma with carcinoma in situ transformation that was completely excised. The median radiation dose was 60 Gy. Eight patients received chemotherapy either concurrent with radiation therapy (5 patients), as neoadjuvant treatment (1 patient), or for progressive or metastatic disease (3 patients). The median follow-up time was 27 months (range, 6-96 months). In
10 patients, the globe was spared. In 9 of these 10 patients, visual acuity was the same as at baseline or better than 20/40 at last follow up.\n\nConclusions: With multidisciplinary therapy, the eye can be spared and reasonable visual function can be preserved in most patients with primary lacrimal sac/nasolacrimal duct carcinomas.”
“Objective: To investigate experimentally the time dependent changes of latency, amplitude, threshold of neural response in injured rat facial nerve in a nerve-crush trauma model.\n\nMaterials and Methods: Thirty Wistar rats weighing 220-280 g (12-16 week), were grouped for permanent and transient nerve injury during time course analysis of electrophysiological changes at 1st week, and 1st, 3rd and 6th months.
These functionally different iNKT subsets segregate
in their ability to bind CD1d-tetramers loaded with the partial agonist alpha-linked glycolipid antigen OCH and structurally different endogenous beta-glycosylceramides. Using surface plasmon resonance with recombinant iNKT TCRs and different ligand-CD1d complexes, we demonstrate that the CDR3 beta sequence strongly impacts on the iNKT TCR affinity to CD1d, independent of the loaded AP26113 chemical structure CD1d ligand. Collectively our data reveal a crucial role for CDR3 beta for the function of human iNKT cells by tuning the overall affinity of the iNKT TCR to CD1d. This mechanism is relatively independent of the bound CD1d ligand and thus forms the basis of an inherent, CDR3 beta dependent functional hierarchy of human iNKT cells.”
“Genetic testing for BRCA1 and BRCA2 mutations is gaining acceptance LY2157299 concentration in clinical oncology worldwide and may help target unaffected high-risk women for prevention and for close surveillance. Annual screening with MRI seems to be an effective surveillance strategy, but the long term follow-up of women with small MRI-detected breast cancers is necessary to establish its ultimate value. Women with cancer and a BRCA mutation may benefit from
tailored treatments, such as cisplatin or olaparib. The treatment goals for a woman with a BRCA-associated breast cancer should be to prevent recurrence of the initial cancer and to prevent second primary breast and ovarian cancers. Mutations in BRCA1 and BRCA2 are presented throughout the world and it is important that the benefits of genetic testing and of targeted therapies be extended to women
who live outside of North America and Western Europe.”
“The viral haemorrhagic septicaemia virus (VHSV) comprises 4 major genotypes and a number of subtypes with, in most cases, distinct geographical distribution. A quick and simple detection method that can discriminate the different genotypes is desirable for a quick and more efficient prevention of the spread of genotypes to new geographical CX-6258 areas. A monoclonal antibody (MAb) against VHSV genotype IVa was produced, with the aim of providing a simple method of discriminating this genotype from the other VHSV genotypes (I, II, III and IVb). Balb/c mice were injected with purified VHSV-JF00Ehil (genotype IVa) from, diseased farmed Japanese flounder. Ten hybridoma clones secreting monoclonal antibodies (MAbs) against VHSV were established. One of these, MAb VHS-10, reacted only with genotype IVa in indirect fluorescent antibody technique (IFAT) and ELISA. Using cell cultures that were transfected with each of the viral protein genes, it was shown that the MAb VHS-10 recognizes a nonlinear genotype IVa-specific epitope on the VHSV N-protein.”
“Premise of research.
5 g.kg(-1) body mass of CIT or placebo (PLC) in 1000 mL of solution. These trials were separated by 2 weeks. Baseline (before ingestion) aldosterone concentration did not differ between the 2 trials; however, it was 36.5% (p = 0.003) lower in the CIT trial compared with the PLC trial before the running test (i.e., after ingestion). The extent of the running-induced increase in
aldosterone was 33% (p = 0.009) smaller in the CIT trial. There were no between-trial differences in the levels of adrenocorticotropic hormone, BV-6 cell line N-terminal pro-B-type natriuretic peptide, or renin activity at any stage of the study. However, a greater relative increase in plasma volume (mean +/- SD, 6.41% +/- 3.78% vs. 4.08% +/- 3.33%; p = 0.042) was Wnt inhibitor observed after administering the CIT compared with the PLC drink. Serum Na(+) concentration increased (by 3.1 +/- 1.2 mmol.L(-1); p < 0.0001) after ingestion of the CIT but not the PLC drink. A higher Na(+) level was observed in the CIT trial than in the PLC trial (142.4 +/- 1.6 vs. 139.3 +/- 1.4 mmol.L(-1), p = 0.00001) after completion of the run. In conclusion,
pre-exercise ingestion of CIT induces a decrease in serum aldosterone concentration in the resting condition and a blunting of the aldosterone response during incremental running exercise to volitional exhaustion. The observed effect of CIT on the serum aldosterone level may be mediated by an acute increase in plasma volume and serum Na+ concentration alterations.”
“A 36-year-old woman presented to our hospital with a short history of intermittent headaches. All MRI of the brain
revealed a left temporal intracerebral cystic lesion with rim enhancement. Histopathology showed a malignant tumour With features of rhabdoid differentiation. Immunohistochemistry revealed that vimentin, epithelial membrane antigen and S-100 were positive, and that glial fibrillary acidic Protein and the chromosome deletion 1p/19q were negative. The patient was diagnosed as having an intracerebral cystic rhabdoid meningioma. She was treated With surgery and post-operative radiotherapy. Cystic intracerebral rhabdoid meningiomas are rare. We discuss the clinical picture of this patient with reference Ruboxistaurin to the published literature on this Uncommon diagnosis. (C) 2008 Elsevier Ltd. All rights reserved.”
“Sexually transmitted diseases (STD) are probably the most tabooed diseases we know. The many taboos and the related stigmata shape patients’ lives and significantly influence health care policies, medical research, and current problems in medical ethics. To better understand these complex influences, the still powerful taboos and related metaphors associated with illness and disease are analyzed within their cultural and historical background and concerning the actual impact on patient care and research.
6-7.6, and the influence of other differently charged proteins (apomyoglobin, egg lysozyme, lactalbumin, and BSA) has been studied at pH 7.4. It is shown that the rate of mitochondrial respiration in the presence of MbO(2) increases by 10-30% (V (1) > V (0)). No myoglobin effect is observed for FCCP-uncoupled MC (V (max) does not change). The rate of MbO(2) deoxygenation is equal to the rate of oxygen uptake by mitochondria (V (2)/V (1) similar to 1 at pH 7.2-7.5). At varying pH < 7.2, the V (2) values become markedly higher than V (1), evidently due Apoptosis inhibitor to
the increased MbO(2) positive charge and its stronger interaction with negatively charged mitochondrial membrane. At pH 7.4, on the contrary, V (2) is twice lower than V (1) in the case of negatively charged CM-MbO(2) (pI
5.2), which has carboxymethylated histidines. Positively charged lysozyme (pI 11) strongly inhibits MbO(2) deoxygenation (V (2)) without affecting oxygen uptake by MC (V (0) and V (1)). At the same time, apomyoglobin (pI 8.5), which is structurally very similar to the holoprotein, and both negatively charged lactalbumin (pI 4.4) and BSA (pI 4.7) have no substantial influence on V (2) and V (1). The MC membrane evidently has no specific sites for the interaction with myoglobin. Rather, the protein contacts with phospholipids of the outer membrane during MbO(2) deoxygenation, and electrostatic interactions are of great importance for this process.”
“A GSK J4 mw compelling example of auditory-visual multisensory integration AZD6738 manufacturer is the McGurk effect, in which an auditory syllable is perceived very differently depending on whether it is accompanied by a visual movie of a speaker pronouncing the same syllable or a different, incongruent syllable. Anatomical and physiological studies in human and nonhuman primates have suggested that the superior temporal sulcus (STS) is involved in auditory-visual integration for both speech and nonspeech stimuli.
We hypothesized that the STS plays a critical role in the creation of the McGurk percept. Because the location of multisensory integration in the STS varies from subject to subject, the location of auditory-visual speech processing in the STS was first identified in each subject with fMRI. Then, activity in this region of the STS was disrupted with single-pulse transcranial magnetic stimulation (TMS) as subjects rated their percept of Mc Gurkand non-McGurk stimuli. Across three experiments, TMS of the STS significantly reduced the likelihood of the McGurk percept but did not interfere with perception of non-McGurk stimuli. TMS of the STS was effective at disrupting the McGurk effect only in a narrow temporal window from 100 ms before auditory syllable onset to 100 ms after onset, and TMS of a control location did not influence perception of McGurk or control stimuli. These results demonstrate that the STS plays a critical role in the McGurk effect and auditory-visual integration of speech.
\n\nResults. The C-13-MBT differed significantly between healthy controls and cirrhotic patients at all time intervals measured. It also proved the ability to differentiate patients with liver cirrhosis based on severity of hepatic impairment corresponding to the Child-Pugh classification A vs. B vs. C. The ROC curve analysis suggested that the best prediction is provided by time intervals between the 10th – 20th
or 10th – 40th minute of PDR.\n\nConclusions. The C-13-MBT offers a reliable means for quantification of hepatic metabolic function over the complete range of functional liver impairment. It is non-invasive, easy to perform and completely safe.”
“PURPOSE CH5424802 purchase ACY-1215 research buy To describe optical coherence tomography (OCT) findings of ocular lesions in pseudoxanthoma elasticum (PXE).\n\nMETHODS. Sixteen eyes of 8 patients with PXE and 20 eyes of 10 age-matched healthy volunteers were included in the study. All patients in the study and control group underwent a complete ophthalmologic examination and OCT. Fluorescein angiography was performed on the patients with PXE.\n\nRESULTS. In the areas of peau de orange mottling, OCT demonstrated increased reflectivity on the level of retinal pigment epithelium (RPE), Bruch membrane, and choriocapillaris complex. OCT scans of crystalline
body showed a hyperreflective shell and an isoreflective or hyporeflective core. OCT scans of the angioid streaks demonstrated thinning in RPE-Bruch
membrane-choriocapillaris complex.\n\nCONCLUSIONS. Disturbances on the 4EGI-1 purchase level of RPE, choroid, and Bruch membrane may be responsible for the ocular lesions in PXE. OCT may give clues to the pathophysiology of the retinal lesions. Spectral domain OCT could provide more details and information. Further studies using this new technology should be performed. (Eur J Ophthalmol 2010; 20: 397-401)”
“Using a microfluidic chip, we have investigated whether bone marrow mesenchymal stem cells (BM-MSCs) could ameliorate IL-1 beta/IFN-gamma-induced dysfunction of INS-1 cells. BM-MSCs were obtained from diabetes mellitus patients and their cell surface antigen expression profiles were analyzed by flow cytometric. INS-1 cells were cocultured with BM-MSCs on a microfluidic chip with persistent perfusion of medium containing 1 ng/mL IL-1 beta and 2.5 U/mL IFN-gamma for 72 h. BM-MSCs could partially rescue INS-1 cells from cytokine-induced dysfunction and ameliorate the expression of insulin and PDX-1 gene in INS-1 cells. Thus BM-MSCs can be viewed as a promising stem cell source to depress inflammatory factor-induced dysfunction of pancreatic beta cells in diabetic patients.