pylori-infected dyspeptic patients (44 non-ulcer, 23 peptic ulcer, 22 gastric cancer) were included in the study. Patients were considered to have H. pylori infection when at least one invasive method (i.e., culture, rapid urease
test, and histology on biopsy specimens) and serological tests including a commercial ELISA (Pyloriset EIA-GIII) and a commercial immunoblot (Helicoblot 2.1; Genelabs Diagnostics), were positive. In addition, the sera of 20 H. pylori-infected blood donors and 10 H. pylori-non-infected blood donors were also randomly learn more collected and analyzed for H. pylori infection by ELISA and Helicoblot 2.1.
Results: Immunoreactive protein bands at 116-kDa, 89-kDa, 37-kDa, 35-kDa, 30-kDa, 19.5-kDa, and the current
infection marker for H. pylori-infected patients had average frequencies of 97.8%, 77.5%, 36.0%, 25.8%, 79.8%, 58.4%, and 69.7%, respectively. see more The immunoreactive patterns obtained from the H. pylori-infected patients and H. pylori-infected blood donors were similar. The antibodies to VacA and CagA antigens were not significantly different among the H. pylori-infected gastroduodenal patient groups. The simultaneous presence of antibody to 19.5-kDa antigen and absence of antibody to 35-kDa antigen was associated with an increased risk of gastric cancer (p < 0.05). The immunoreactive band to 35-kDa antigen was found at significantly higher levels in peptic ulcer patients, and the 37-kDa antigen was found at
significantly higher levels in non-ulcer patients (both p < 0.05). Significantly low Levels of antibodies to 23-kDa and 85-kDa antigens were found associated with peptic ulcer (p < 0.05).
Conclusions: We confirm that the universal presence of CagA and VacA in H. pylori-infected patients in Thailand is independent of the gastroduodenal disease. The presence or absence of antibodies to H. pylori-specific antigens may be useful as indirect markers in the screening of H. pylori-infected patients, and may have specific protection roles in H. pylori-related gastroduodenal diseases. (C) 2009 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.”
“A viscosimetric method has been used to study the interpolymer association between poly(vinyl alcohol) (PVA) and poly(sodium styrene sulfonate) (PSSNa) in aqueous solution. At constant molecular ACY-738 datasheet weight of PSSNa, it was found that, the PVA and PSSNa associations were improved with the decrease of molecular weight of PVA and the decrease of its hydrolysis degree. The measurement of intrinsic viscosity [eta] and the determination of Huggins associative coefficient K(H) of different PVA samples were used to select the most appropriate PVA sample, which leads to homogeneous polymer-polymer mixtures (PVA with hydrolysis degree 87-89%, molecular weight 124,000-186,000 g/mol, intrinsic viscosity [eta] 1.02 dL/g, and Huggins associative coefficient K(h.ass) = 0.76).